橄榄绿链霉菌E-86产木聚糖酶水解玉米芯汽爆液生产低聚木糖

食品科学 ›› 2004, Vol. 25 ›› Issue (8): 87-90.

橄榄绿链霉菌E-86产木聚糖酶水解玉米芯汽爆液生产低聚木糖

李里特, 艾志录, 江正强, 朱运平, 张宁

中国农业大学食品科学与营养工程学院

出版日期:2004-08-15 发布日期:2011-10-24

Xylanase of Streptomyces olivaceovirdis E-86 Used to Hydrolyze Steam-exploded Corncob Extract for Xylooligosaccharides Production

LI Li-Te, AI Zhi-Lu, JIANG Zheng-Qiang, ZHU Yun-Ping, ZHANG Ning

College of Food Science and Nutritional Engineering, China Agricultural University

Online:2004-08-15 Published:2011-10-24

摘要/Abstract

摘要： 本文以橄榄绿链霉菌E-86产木聚糖酶水解玉米芯汽爆液生产低聚木糖为目的,研究了玉米芯汽爆液的水解特征,并与玉米芯木聚糖的酶解产物组成进行了比较,得到如下结果:加酶量120U/100ml、酶解反应8h可获得较好的酶解效果,直接还原糖量46μmol/ml、平均聚合度3.1、水解率46%;玉米芯汽爆液和玉米芯木聚糖的酶解产物中低聚糖组成大致相同,主要是木二糖和少量的木三糖、木糖,汽爆液酶解产物中还含有极少量的鼠李糖和阿拉伯糖;玉米芯汽爆液可代替玉米芯木聚糖为底物生产低聚木糖。

关键词: 玉米芯汽爆液, 低聚木糖生产, 木聚糖酶, 橄榄绿链霉菌E-86

Abstract: Xylanase produced by Streptomyces olivaceovirdis E-86 was used to hydrolyze steam-exploded corncob extractto produce xylooligosaccharides. Through this experiment, the hydrolysis nature of steam-exploded corncob extract wascharacterized and the composition of its end-products was compared to those of enzymatic hydrolyzed corncob xylan. Thefollowing results were obtained: Satisfactory hydrolysis was achieved at 120U/100ml of enzyme addition and 8h of reactiontime. The amount of reducing sugar in the hydrolysate was 46μmol/ml with average degree of polymerization of 3.1 and hydrolysisrate of 46%. The end-product composition of steam-exploded corncob extract and corncob xylan were almost the same, whichmade up of mainly xylobiose and small amounts of xylotriose and xylose. A trace amount of rhamnose and arabinose were alsofound in the hydrolysate of steam-exploded corncob extract. Thus, the steam-exploded corncob extract was appropriate toreplace corncob xylan as the substrate for xylooligosaccharides production.

Key words: steam-exploded corncob, xylooligosaccharides production, xylanase, Streptomyces olivaceovirdis E-86

李里特, 艾志录, 江正强, 朱运平, 张宁. 橄榄绿链霉菌E-86产木聚糖酶水解玉米芯汽爆液生产低聚木糖[J]. 食品科学, 2004, 25(8): 87-90.

LI Li-Te, AI Zhi-Lu, JIANG Zheng-Qiang, ZHU Yun-Ping, ZHANG Ning. Xylanase of Streptomyces olivaceovirdis E-86 Used to Hydrolyze Steam-exploded Corncob Extract for Xylooligosaccharides Production[J]. FOOD SCIENCE, 2004, 25(8): 87-90.

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