食品科学

• 生物工程 • 上一篇    下一篇

单宁酸对纤维素酶和木聚糖酶活力的影响

田 野,欧仕益   

  1. 暨南大学食品科学与工程系,广东 广州 510632
  • 出版日期:2013-06-15 发布日期:2013-06-03

Influence of Tannic Acids on the Activities of Cellulase and Xylanase

TIAN Ye,OU Shi-yi   

  1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China
  • Online:2013-06-15 Published:2013-06-03

摘要:

利用酶动力学、紫外和荧光光谱法,研究单宁酸对纤维素酶和木聚糖酶活力的影响。结果表明:单宁酸抑制2种酶活性,对木聚糖酶和纤维素酶的抑制质量浓度分别为0.5mg/mL和1.0mg/mL,抑制率分别为37.16%和35.33%。动力学研究表明:单宁酸对纤维素酶具有竞争性抑制作用;而对木聚糖酶的抑制作用表现为提高其亲和力,但降低酶反应速率。紫外和荧光光谱研究表明,单宁酸能与酶蛋白中的芳香氨基酸残基发生共价反应,生成稳定化合物,从而影响酶反应。

关键词: 单宁酸, 纤维素酶, 木聚糖酶, 酶活力

Abstract:

The effect of tannic acid on the activities of cellulase and xylanase was investigated and the mechanisms were
explored using enzymatic kinetics, ultroviolet spectroscopy and fluorescence spectroscopy. Tannic acid was found to inhibit
both enzymes with an inhibitory rate of 0.5 mg/mL (37.16%) for xylanase and 1.0 mg/mL(35.33%) for cellulase. Kinetic
invesitgations illustrated that taannic acid had competitve inhibition on cellulase, while its inhibition on xylanase was
manifested by improving its affinity but decreasing the reaction rate. UV and fluorescence spectroscopic analyses suggested
that covalent interactions of tannic acid with the aromatic amino acid residues in enzyme molecules formed stable complexes
so as to influence the enzyme reactions.

Key words: tannic acid, cellulase, xylanase, activity