高压脉冲电场协同酶法辅助提取玫瑰精油工艺优化

doi:10.7506/spkx1002-6630-20191104-042

食品科学 ›› 2020, Vol. 41 ›› Issue (6): 270-277.doi: 10.7506/spkx1002-6630-20191104-042

高压脉冲电场协同酶法辅助提取玫瑰精油工艺优化

周亚军，李圣桡，王淑杰，薛长美，姚光明

（1.吉林大学食品科学与工程学院，吉林长春 130062；2.吉林大学生物与农业工程学院，吉林长春 130025；3.盘锦牙博士口腔门诊部，辽宁盘锦 124000）

收稿日期:2020-03-26 修回日期:2020-03-26 出版日期:2020-03-25 发布日期:2020-03-23

Optimization of Extraction of Rose Essential Oil by High Intensity Pulsed Electric Field Combined with Enzymatic Treatment-Assisted Steam Distillation

ZHOU Yajun, LI Shengrao, WANG Shujie, XUE Changmei, YAO Guangming

(1. College of Food Science and Engineering, Jilin University, Changchun 130062, China;2. College of Biological and Agriculture Engineering, Jilin University, Changchun 130025, China;3. Panjin Dentist Stomatology Department, Panjin 124000, China)

Received:2020-03-26 Revised:2020-03-26 Online:2020-03-25 Published:2020-03-23

摘要/Abstract

摘要： 为了寻求玫瑰精油的高效提取方法，采用高压脉冲电场（high intensity pulsed electric field，PEF），协同纤维素酶辅助提取玫瑰精油。通过单因素试验、对比实验和Box-Behnken设计试验对玫瑰精油提取工艺优化，并利用气相色谱-质谱联用技术对其进行成分分析。结果表明，PEF协同酶法辅助提取玫瑰精油得率比水蒸气蒸馏和酶辅助水蒸气蒸馏提取法分别提高了约43.83%和20.48%，总耗时分别节省了25%和40%；PEF协同酶法辅助提取玫瑰精油最优提取条件为蒸馏时间1.7 h、脉冲数10 个、电场强度21 kV/cm，在此条件下玫瑰精油得率为0.116 5%；从PEF协同酶法辅助提取玫瑰精油中共鉴定出63 种成分，占精油总量的95.04%，PEF协同酶法处理能够促进精油成分释放。PEF协同酶法辅助提取是一种玫瑰精油的高效提取方法。

关键词: 高压脉冲电场, 玫瑰精油, 提取, 纤维素酶

Abstract: In order to explore a high-efficiency method for extracting rose essential oil, the extraction of rose essential oil by combined use of high intensity pulsed electric field (PEF), celluase hydrolysis and steam distillation was optimized by one-factor-at-a-time method and response surface methodology coupled with Box-Behnken. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that compared with single and enzyme-assisted steam distillation extraction, PEF followed by enzyme-assisted steam distillation increased the oil yield by about 43.83% and 20.48%, and shortened the extraction time by 25% and 40%, respectively. The optimal parameters of PEF followed by enzyme-assisted steam distillation were obtained as follows: distillation time 1.7 h, pulse number 10, and electric field strength 21 kV/cm. Under these conditions, the oil yield was 0.116 5%. A total of 63 components were identified from the essential oil, accounting for 95.04% of the total essential oil. PEF combined with enzymatic treatment could promote the release of essential oil components and as a result, provide an efficient method for the extraction of rose essential oil.

Key words: high intensity pulsed electric field, rose essential oil, extraction, celluase

中图分类号:

TQ654.2

周亚军，李圣桡，王淑杰，薛长美，姚光明. 高压脉冲电场协同酶法辅助提取玫瑰精油工艺优化[J]. 食品科学, 2020, 41(6): 270-277.

ZHOU Yajun, LI Shengrao, WANG Shujie, XUE Changmei, YAO Guangming. Optimization of Extraction of Rose Essential Oil by High Intensity Pulsed Electric Field Combined with Enzymatic Treatment-Assisted Steam Distillation[J]. FOOD SCIENCE, 2020, 41(6): 270-277.

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高压脉冲电场协同酶法辅助提取玫瑰精油工艺优化

Optimization of Extraction of Rose Essential Oil by High Intensity Pulsed Electric Field Combined with Enzymatic Treatment-Assisted Steam Distillation

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