关键词: 纤维素酶；异源表达；结构域融合；生物膜清除

Abstract: In this study, a novel cellulase gene, CelL7, was cloned from the marine bacterium Zobellia sp. B2. Furthermore, a fusion gene, CelL7-CBM3, was constructed by fusing a carbohydrate-binding module family 3 (CBM3) to CelL7 and heterologously expressed in Escherichia coli BL21. The expressed fusion protein was purified by affinity column chromatography. The full length of the CelL7 gene was 1 077 bp, encoding 358 amino acid residues, and the theoretical molecular mass of the encoded protein was 40.39 kDa. The specific enzyme activities of CelL7 and CelL7-CBM3 were 2 249.81 and 2 915.75 U/mg, respectively. The optimal reaction temperatures for both enzymes were 50 ℃, and the optimal pHs were 5.0 and 5.5, respectively. Mn2+ and Fe2+ activated the activity of CelL7, while Cu2+ inhibited it. CelL7 was capable of degrading carboxymethyl cellulose sodium, cellobiose, and xylan. When sodium carboxymethyl cellulose was used as a substrate, the Michaelis-Menten constant (Km) of CelL7-CBM3 was 11.70 mg/mL, which was lower than that of CelL7 (Km = 13.23 mg/mL), indicating that the fusion enzyme, with an added binding domain, exhibited enhanced affinity for carboxymethyl cellulose sodium. The maximum reaction rate (Vmax) was 175.44 mg/(mL·min), the catalytic constant (Kcat) was 2.78 s-1, and the Kcat/Km was 0.24 mL/(mg·s), which were comparable to those of CelL7. Biofilm clearance experiments showed that concentrations of CelL7 ranging from 10.0 to 60.0 μg/mL and those of CelL7-CBM3 ranging from 30.0 to 60.0 μg/mL were effective in dispersing biofilm and reducing the amount of biofilm.

Key words: cellulase; heterologous expression; domain fusion; biofilm eradication