关键词: β-1,3-葡聚糖酶；糖苷水解酶；结构与功能；催化机制；应用

Abstract: β-1,3-Glucanases are enzymes that specifically hydrolyze β-1,3-glycosidic linkages bonds in β-1,3-glucan to generate a range of oligosaccharides or monosaccharides. β-1,3-Glucanases have important potential applications in functional oligosaccharide preparation, fruit and vegetable preservation, biopharmaceuticals, and plant disease resistance. β-1,3-Glucanases belonging to 12 glycoside hydrolase (GH) families have been identified, including GH16, GH17, GH55, GH64, GH81, GH128 and GH132. β-1,3-Glucanases are widely distributed in bacteria, fungi, plants and insects, which exhibit diverse structures and catalytic functions due to differences in sources and sequence evolution. Structural and functional studies of enzymes are the basis for exploring the catalytic mechanism, enzyme properties, and molecular modification. Therefore, this paper reviews the current state of research on the structure, function and application of β-1, 3-glucanases, in order to provide a reference for the basic research and application of β-1,3-glucanases.

Key words: β-1,3-glucanases; glycoside hydrolases; structure and function; catalytic mechanism; application