食品科学 ›› 2004, Vol. 25 ›› Issue (8): 73-75.

• 基础研究 • 上一篇    下一篇

香蕉多糖的分离纯化及其性质研究

 沈建林, 余龙江, 金文闻, 沈红元, 朱涛   

  1. 华中科技大学生命科学与技术学院
  • 出版日期:2004-08-15 发布日期:2011-10-24

Isolation, Purification and Characteristics Study on Banana Polysaccharide

 SHEN  Jian-Lin, YU  Long-Jiang, JIN  Wen-Wen, SHEN  Hong-Yuan, ZHU  Tao   

  1. College of Life Science and Technology, Huazhong University of Science and Technology
  • Online:2004-08-15 Published:2011-10-24

摘要:  本文建立了香蕉多糖的提取纯化方法,并对提取的香蕉多糖进行了理化性质分析。香蕉多糖经40%乙醇分级得到的组分Len5,用Seohadex G-150凝胶柱层析得到单一峰,Len5 的酸性水解液经纸层析和薄层层析分析,其单糖组成为葡萄糖和木糖,用Sephadex G-150 凝胶柱层析法测得其分子量为43000道尔顿。

关键词: 香蕉, 多糖, 分离纯化, 理化性质

Abstract: In this study, a method of banana polysaccharide isolation and purification was set up and the physical and chemicalcharacteristics of banana polysaccharide was also analyzed. Product Lens was obrained when banana polysaccharide wasclassified with 40% ethanol, and a single apex was obtained in the Sephadex G-150 gel chromatogaphy analysis.The monosac-charide components of the acidic solution of Lens5 were identified as glucose and xylose according to the results of paperchromatography and thin-layer chromatography analysis. The molecular weight of Len5 is 43000 Dalton according to the resultof Sephadex G-150 gel chromatography analysis.

Key words: banana, polysaccharide, isolation and purification, physical and chemical characteristic