食品科学 ›› 2007, Vol. 28 ›› Issue (6): 252-255.

• 生物工程 • 上一篇    下一篇

温度、pH、药物对大肠杆菌抑制作用的量热法研究

李向阳, 邵卫华, 刁恩杰, 张洪林   

  1. 山东农业大学食品科学与工程学院; 山东省威海市统一路学校; 曲阜师范大学化学系 山东泰安271018; 山东威海264200; 山东泰安271018; 山东曲阜273165;
  • 出版日期:2007-06-15 发布日期:2011-12-31

Inhibition Study on E.coli by Temperature,pH and Natural Drug with Microcalorimetric Method

 LI  Xiang-Yang, SHAO  Wei-Hua, DIAO  恩Jie, ZHANG  Hong-Lin   

  1. 1.College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China;2.Weihai Tongyi School, Weihai 264200, China;3.Department of Chemistry, Qufu Normal University, Qufu 273165, China
  • Online:2007-06-15 Published:2011-12-31

摘要: 本实验用热活性检测系统测定了大肠杆菌在不同温度、pH及药物的作用下生长的完整热谱曲线。进而按指数生长模型计算出不同条件下细菌代谢的生长速率常数,并用计算机拟合出生长速率常数与温度、pH及药物浓度之间的关系式,进一步求得大肠杆菌最适生长温度,最低和最高生长温度;最佳生长pH及最低和最高生长pH;甘草液抑菌的最佳浓度。此研究工作的开展对深入研究细菌生长规律及抑制细菌生长提供了一种新的可靠的定量方法。

关键词: 大肠杆菌, 微量量热法, 生长速率常数, 抑菌

Abstract: In this experiment, the growth rate constants of E.coli were assayed with microcalorimetric method by different temperatures, pHs and glycyrrhiza concentrations. Then, the relation among rate constant, temperatures, pHs and drug concentrations were simulated by computer. By this means, the optimum temperature for growth is obtained as 311K(38 ℃). At the same time, the optimum pH for growth is 7.79 and the optimum concentration of glycyrrhiza 0.192 g/ml.

Key words: E.coli, microcalorimetric method, growth rate constants, glycyrrhiza