食品科学 ›› 2002, Vol. 23 ›› Issue (4): 51-53.

• 工艺技术 • 上一篇    下一篇

酶法水解珍珠贝肉蛋白质的工艺探讨

 郝记明, 张静, 谌素华, 洪鹏志, 章超桦   

  • 出版日期:2002-04-15 发布日期:2012-02-13

Probe into Enzyme Method of Pinctada Martensii Hydrolysis

 HAO  Ji-Ming, ZHANG  Jing, CHEN  Su-Hua, HONG  Peng-Zhi, ZHANG  Chao-Hua   

  • Online:2002-04-15 Published:2012-02-13

摘要: 研究了用双酶水解技术对马氏珠母贝肉进行水解的条件。结果表明:枯草杆菌中性蛋白酶:在pH6.5、温度40℃、酶浓度3×103U/ml、K+浓度0.12%、水解时间3.0h后;再用胃蛋白酶水解:pH3.0,温度60℃,酶浓度4.5U/ml、水解时间3.0,最适底物浓度为原料与水比例1:3进行水解,蛋白质水解率为66.2%。水解液可用于生产海鲜调味料及功能性蛋白饮料。

关键词: 马氏珠母贝, 酶法水解

Abstract: In this paper ,we studied useing proteinase to hydrolyze Pinctada martensii meat. The optimal conditions for hydrolysis by enzyme method were : B.Subctilis neutral proteinase hydrolyzed at pH6.5,temperature 40℃, enzyme consistency 3×103U/ml , K+ concentraction 0.12%,and time 3.0h.Then add stomach proteinase at pH3.0,temperature 60℃, enzyme consistency 4.5U/ml , and time 3.0h, The degree of hydrolysis of mussel meat is 66.2% by this method. The hydrolysis liquid can be used to make high quality seafresh seasoner and fundational protein drink.

Key words: Pinctada martensii , Hydrolysis by enzyme method