高产二十二碳六烯酸隐甲藻的选育及其发酵条件研究

doi:10.7506/spkx1002-6630-201305042

食品科学 ›› 2013, Vol. 34 ›› Issue (5): 202-206.doi: 10.7506/spkx1002-6630-201305042

高产二十二碳六烯酸隐甲藻的选育及其发酵条件研究

孙中贯1,2，刘咏1,*，姚建铭3，李俊4，朱素梅4

1.合肥工业大学生物与食品工程学院，安徽合肥 230009；2.枣庄学院生命科学学院，山东枣庄 277160； 3.中国科学院等离子体物理研究所，安徽合肥 230031；4.安徽科聚环保新能源有限公司，安徽合肥 230031

收稿日期:2011-11-17 修回日期:2013-01-17 出版日期:2013-03-15 发布日期:2013-04-16
通讯作者: 刘咏 E-mail:liuy99999@163.com

Screening and Breeding of a Crypthecodinium cohnii Strain with High Productivity of Docosahexaenoic Acid and Optimization of Its Fermentation Conditions

SUN Zhong-guan1,2，LIU Yong1,*，YAO Jian-ming3，LI Jun4，ZHU Su-mei4

1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China；2. College of Life Science, Zaozhuang University of Technology, Zaozhuang 277160, China；3. Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China；4. Anhui Keju Green Energy Co. Ltd., Hefei 230031, China

Received:2011-11-17 Revised:2013-01-17 Online:2013-03-15 Published:2013-04-16
Contact: LIU Yong E-mail:liuy99999@163.com

摘要/Abstract

摘要： 利用亚硝基胍对隐甲藻ATCC30556进行诱变处理，通过30℃高温培养初筛及摇瓶发酵复筛，得到二十二碳六烯酸(DHA)产量稳定提高的突变株LS1057，其DHA占总脂肪酸的含量从出发株的25.78%提高到32.02%。研究摇瓶发酵条件对突变株发酵的影响，结果表明：在初始pH6.5、温度26℃、转速150r/min的发酵条件下培养216h后，其生物量、总油脂产量及DHA产量分别达到14.06、3.14g/L和1.057g/L，分别比出发藻株提高了21.31%、69.73%和121.59%。

关键词: 二十二碳六烯酸, 亚硝基胍, 隐甲藻, 诱变育种, 发酵条件

Abstract: In order to improve the productivity of docosahexaenoic acid, the mutagenesis was carried out through nitrosoguanidine (NTG) induction by using Crypthecodinium cohnii ATCC30556 as the original strain. The mutant LS1057 was selected through high-temperature (30 ℃) cultivation using docosahexaenoic acid content in total fatty acids as the evaluation index. The content of docosahexaenoic acid was increased from 25.78% to 32.02%. The fermentation conditions for LS1057 were optimized by single-factor tests to be pH 6.5, fermentation temperature of 26 ℃, shaking speed of 150 r/min and cultivation time of 216 h. Under the optimal conditions, the biomass of cultivated LS1057 could reach up to 14.06 g/L with total lipid of 3.14 g/L and docosahexaenoic acid of 1.057 g/L, which exhibited the enhancement by 21.31%, 69.73% and 121.59% when its parent strain.

Key words: docosahexaenoic acid (DHA), nitrosoguanidine, Crypthecodinium cohnii, mutagenesis, fermentation conditions

中图分类号:

TS202.3

孙中贯1,2，刘咏1,*，姚建铭3，李俊4，朱素梅4. 高产二十二碳六烯酸隐甲藻的选育及其发酵条件研究[J]. 食品科学, 2013, 34(5): 202-206.

SUN Zhong-guan1,2，LIU Yong1,*，YAO Jian-ming3，LI Jun4，ZHU Su-mei4. Screening and Breeding of a Crypthecodinium cohnii Strain with High Productivity of Docosahexaenoic Acid and Optimization of Its Fermentation Conditions[J]. FOOD SCIENCE, 2013, 34(5): 202-206.

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高产二十二碳六烯酸隐甲藻的选育及其发酵条件研究

Screening and Breeding of a Crypthecodinium cohnii Strain with High Productivity of Docosahexaenoic Acid and Optimization of Its Fermentation Conditions

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