刺玫叶中金丝桃苷和总黄酮含量的测定

食品科学 ›› 2013, Vol. 34 ›› Issue (2): 231-235.

刺玫叶中金丝桃苷和总黄酮含量的测定

王晓林¹,钟方丽²,孙晓雨¹,王建刚²

1. 吉林市龙潭区承德街45号吉林化工学院
2. 吉林化工学院

收稿日期:2011-08-05 修回日期:2012-07-23 出版日期:2013-01-25 发布日期:2013-01-15
通讯作者: 王晓林 E-mail:wangxiaolin69@eyou.com
基金资助:
吉林省教育厅重点计划项目

Content Determination of Hyperoside and Total Flavonoids in Leaves of Rosa davurica Pall.

Received:2011-08-05 Revised:2012-07-23 Online:2013-01-25 Published:2013-01-15

摘要/Abstract

摘要：

摘要目的：建立测定刺玫叶中金丝桃苷和总黄酮含量的方法。方法：以芦丁为对照品，采用紫外分光光度法于418nm测定总黄酮含量；采用高效液相色谱法测定金丝桃苷含量，采用Shim-pack VP-ODS C18色谱柱(150mm×4.6mm,5μm)，流动相为甲醇-0.5%磷酸(45:55) 溶液，检测波长为360 nm，流速为1.0 mL/min，柱温40℃。结果：总黄酮在0.02～0.12 mg/mL（R=0.9996）、金丝桃苷在0.192～1.152 μg（R=0.9993）范围内呈良好线性关系；总黄酮和金丝桃苷平均回收率分别为100.87%、100.36%，RSD分别为2.79%和2.26%。结论：所建立的方法快速、准确、操作简便，适用于刺玫叶中金丝桃苷和总黄酮的含量测定。

Abstract:

Abstract: Purpose: To establish the determination method of total flavonoids and hyperoside in leaves of Rosa davurica Pall..Method：UV-spectrophotometric method was applied to determine the content of total flavonoids at the wave length of 418 nm using rutin as reference substance. The content of hyperin was determined with high performance liquid chromatography(HPLC) using Shim-pack VP-ODS C18 (150mm×4.6mm,5μm) column and CH3OH-0.5% H3PO4 (45:55)as gradient elution mobile phase. The UV detection wave-length, the flow rate and the column temperature were 360nm,1.0 mL/min, 40℃ respectively. Result: Total flavonoids and hyperoside have shown good linear relationship in the range of 0.02～0.12mg/mL(R=0.9996)and 0.192~1.152μg(R=0.9993).The average recovery rate and RSD of total flavonoids and hyperoside were100.87%,100.36%, 2.79% and 2.26% respectively. Conclusion: The determination method offered in the paper is characterized with celerity，accuracy and easy operation, which is fit for the content determination of total flavonoids and hyperoside in leaves of Rosa davurica Pall..

中图分类号:

TQ244.2

王晓林钟方丽孙晓雨王建刚. 刺玫叶中金丝桃苷和总黄酮含量的测定[J]. 食品科学, 2013, 34(2): 231-235.

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