食品科学 ›› 2011, Vol. 32 ›› Issue (22): 42-45.doi: 10.7506/spkx1002-6630-201122009

• 工艺技术 • 上一篇    下一篇

芡实叶黄酮超声提取工艺优化

张 然1,2,贺 芳1,崔竹梅1,2,齐 斌1,2,*   

  1. 1.常熟理工学院生物与食品工程学院 2.苏州市食品生物技术重点实验室
  • 出版日期:2011-11-25 发布日期:2011-11-11
  • 基金资助:
    苏州市科学技术局科技项目(SN201035;SYND201001)

Optimization of Ultrasonic Extraction of Flavonoids from Euryale ferox Leaves

Ran1,2,HE Fang1,CUI Zhu-mei1,2,QI Bin1,2,*   

  1. (1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China; 2. Suzhou Key Laboratory of Food and Biotechnology, Changshu 215500, China)
  • Online:2011-11-25 Published:2011-11-11

摘要: 通过Plackett-burman设计法研究料液比、超声功率、乙醇体积分数、提取温度和时间5个因素对芡实叶黄酮提取效果的影响,选择影响效果较显著的4个因素--料液比、超声功率、乙醇体积分数、提取时间通过正交试验优化得出超声提取芡实叶黄酮最佳工艺条件为料液比1:25(g/mL)、超声功率175W、乙醇体积分数85%、提取时间40min,在此条件下芡实叶黄酮提取量为90.28mg/g。

关键词: 芡实叶, 黄酮, 超声波提取

Abstract: In order to maximize extraction efficiency of flavonoids from Euryale ferox leaves, Plackett-Burman design was used to screen 4 main variables (ratio of material to liquid, ultrasonic power, ethanol concentration and extraction time) that influence extraction efficiency out of 5 process parameters (ratio of material to liquid, ultrasonic power, ethanol concentration, temperature and extraction time) and the selected variables were then optimized using orthogonal array design. The optimum conditions for flavonoid extraction from Euryale ferox leaves were determined as follows: 1:25 ratio of material to liquid (g/mL), 175 W ultrasonic power, 85% ethanol as extraction solvent, and 40 min extraction time. Under such conditions, the extraction efficiency of flavonoids was 90.28 mg/g.

Key words: Euryale ferox leaves, flavonoids, ultrasonic extraction

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