离子沉淀法分离连翘槲皮素的工艺条件

食品科学 ›› 2012, Vol. 33 ›› Issue (20): 38-41.

离子沉淀法分离连翘槲皮素的工艺条件

欧阳玉祝1，魏燕1，张曦2，旷超阳1，梁明1

1.吉首大学食品科学研究所 2.湖南省怀化市质量监督局

收稿日期:2011-11-25 修回日期:2012-09-12 出版日期:2012-10-25 发布日期:2012-11-09
通讯作者: 欧阳玉祝 E-mail:ouyang1227@126.com
基金资助:
湖南省科技厅科技计划项目

Optimization of Ion Precipitation Conditions for Separation of Quercetin from Fructus Forsythiae

Received:2011-11-25 Revised:2012-09-12 Online:2012-10-25 Published:2012-11-09

摘要/Abstract

摘要： 用锌离子络合沉淀法分离连翘中的槲皮素，考察沉淀剂用量、沉淀温度和沉淀时间以及碳酸钠用量对槲皮素提取率的影响，用高效液相色谱结合标样对分离产物进行分析。结果表明：在4.00g连翘样品的提取液中，用1.00g无水醋酸锌作沉淀剂，加0.60g碳酸钠，40℃条件下沉淀40min，槲皮素的提取率为4.20%。分离产物的高效液相色谱图与标准品一致。

关键词: 连翘, 槲皮素, 锌离子沉淀法, 分离

Abstract: In order to develop a method for the separation of quercetin from ethanol extract from Fructus Forsythiae, the effects of precipitant dose, temperature and stirring time on quercetin yield were explored. The final product was analyzed by HPLC using quercetin standard. The results showed that the adding 0.60 g of sodium carbonate and 1.00 g of zinc acetate and stirring for 40 min provided a quercetin yield of 4.20% from the 60% ethanol extract from 4.00 g of Fructus Forsythiae. The resulting precipitate showed an HPLC profile similar to that of quercetin standard.

Key words: Fructus Forsythiae, quercetin, zinc ion precipitation method, separation

中图分类号:

TS209

欧阳玉祝1，魏?燕1，张?曦2，旷超阳1，梁?明1. 离子沉淀法分离连翘槲皮素的工艺条件[J]. 食品科学, 2012, 33(20): 38-41.

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