食物过敏原构象性表位鉴别的研究进展

摘要/Abstract

摘要： 构象性表位是表位的重要组成之一，在过敏反应中起重要作用。表位定位工作是过敏原研究的重要领域。目前应用于构象性表位研究的方法主要有3类：基于线性表位的氨基酸组成和特性预测构象性表位；利用噬菌体筛选得到模拟肽，通过生物信息学定位构象性表位以及利用光谱学等方法测定构象性表位与抗体结合的相关参数确定构象型表位。前两种方法比较常用，而光谱学方法是近年来发展的一种新方法，准确性更高。这3类定位方法可为过敏原的研究提供认识和了解构象性表位的途径，并为构象性表位在过敏中的作用机制提供部分理论依据。

关键词: 食物过敏原, 构象性表位, 定位

Abstract: Conformational epitope is an important type of epitope and plays a critical role in allergy. Epitope mapping is the core of allergen research. Today, there are three main conformational epitope mapping approaches, including conformational epitope prediction based on the amino acid composition and characteristics of linear epitopes, epitope mapping by phage display technique and bioinformatics, and conformational epitope mapping by spectroscopic analysis and parameters related to coupling with antibodies. The first two approaches are commonly used in conformational epitope mapping. As a more accurate approach, spectroscopy has not been used in this field until recently. These approaches allow for a better knowledge and understanding of conformational epitopes in allergen research and also provide theoretical evidence for the mechanisms of action of conformational epitopes in allergy.

Key words: food allergen, conformational epitope, mapping

中图分类号:

TS201.6

李欣1,2，陈红兵1,3,*. 食物过敏原构象性表位鉴别的研究进展[J]. 食品科学, 2012, 33(17): 279-283.

Hong-bing CHEN. Research Progress in Conformational Epitope Mapping Approaches for Food Allergens[J]. FOOD SCIENCE, 2012, 33(17): 279-283.

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