嗜热链球菌CH9胞外多糖的分离纯化及结构分析

食品科学 ›› 2012, Vol. 33 ›› Issue (19): 107-112.

嗜热链球菌CH9胞外多糖的分离纯化及结构分析

张新源，刘会平*，曹春玲，季玲

天津科技大学食品工程与生物技术学院，教育部食品营养与安全重点实验室

收稿日期:2011-07-24 修回日期:2012-08-09 出版日期:2012-10-15 发布日期:2012-09-17
通讯作者: 张新源 E-mail:zhangxinyuan628540@163.com
基金资助:
天津科技大学人才引进基金

Purification and Structural Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CH9

Xin-Yuan ZHANG

Received:2011-07-24 Revised:2012-08-09 Online:2012-10-15 Published:2012-09-17
Contact: Xin-Yuan ZHANG E-mail:zhangxinyuan628540@163.com

摘要/Abstract

摘要： 对嗜热链球菌CH9胞外多糖进行分离纯化，经DEAE-52离子交换柱及Sephadex G-100凝胶柱得到单一组分，利用高效液相色谱(HPLC)测其分子质量，结果表明：EPS1a为中性多糖，分子质量为1.8×106u；EPS2a、EPS3a为酸性多糖，分子质量分别为1.06×106、1.05×106u。经琼脂糖凝胶电泳及Sephadex G-100凝胶柱鉴定3种糖均为单一组分。紫外及红外光谱扫描结果显示：3种糖均具有多糖的特征吸收峰，且EPS2a、EPS3a可能为糖与蛋白的复合物。刚果红实验表明：EPS1a具有三股螺旋结构，而其他两种糖并未发现此现象。β消去实验证明：EPS1a、EPS3a为—O—连接，EPS2a为—N—连接。

关键词: 嗜热链球菌, 胞外多糖, 分离纯化, 结构分析

Abstract: Crude exopolysaccharides (EPS) isolated from the fermentation supernatant of Streptococcus thermophilus CH9 were fractionated by DEAE-52 column chromatography into EPS1, EPS2 and EPS3. Sephadex G-100 column chromatography was used to further fractionate the three factions to harvest their respective major components named as EPS1a, EPS2a and EPS3a, respectively. EPS1a was a neutral polysaccharide with a molecular weight of 1.8 × 106 u, and EPS2a and EPS3a were acidic polysaccharides with a molecular weight of 1.06 × 106 u and 1.05 × 106 u, respectively. Agarose gel electrophoresis analysis indicated that all these three polysaccharides consisted of a single component. UV and IR spectroscopic analyses showed that all of them had characteristic adsorption peaks of polysaccharide. EPS2a and EPS3a might be polysaccharide-protein complexes. Congo red test revealed that EPS1a rather than EPS2a and EPS3a had a three-stranded helix structure. Beta elimination test demonstrated that EPS1a and EPS3a were -O-linked, but EPS2a was a -N-linked polysaccharide.

Key words: Streptococcus thermophilus CH9, exopolysaccharides, purification, structural characterization

中图分类号:

TS252

张新源，刘会平*，曹春玲，季?玲 . 嗜热链球菌CH9胞外多糖的分离纯化及结构分析[J]. 食品科学, 2012, 33(19): 107-112.

Xin-Yuan ZHANG. Purification and Structural Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CH9[J]. FOOD SCIENCE, 2012, 33(19): 107-112.

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