复合酶法提取海带多糖工艺优化

食品科学 ›› 2012, Vol. 33 ›› Issue (18): 6-9.

复合酶法提取海带多糖工艺优化

翟为1，张美双1，张莉霞1，赵岩1,*，吴鹏2，张连学1,*

1.吉林农业大学中药材学院 2.九江中星联生物技术有限公司

收稿日期:2011-08-25 修回日期:2012-07-30 出版日期:2012-09-25 发布日期:2012-09-07
通讯作者: 张连学 E-mail:zlx863@163.com
基金资助:
国家科技支撑计划-人参规范化种植提升、系列产品综合开发及品牌培育研究;国家自然科学基金-三萜皂苷类物质对人参种群调节与适应的作用及其机制

Optimization of Multi-Enzyme Hydrolysis of Laminaria japonica for Polysaccharide Extraction

Received:2011-08-25 Revised:2012-07-30 Online:2012-09-25 Published:2012-09-07

摘要/Abstract

摘要： 以海带为原料，复合酶法提取海带多糖。在单因素试验的基础上，采用正交试验及方差分析确定复合酶法提取海带多糖的最佳条件：纤维素酶0.5%、果胶酶1.0%、木瓜蛋白酶1.0%、木聚糖酶1.0%、温度30℃、时间3h、pH 5.0。在此条件下，海带中多糖的提取率为78.9%。使用复合酶前处理工艺，海带多糖产率较传统工艺提高了近3倍。

关键词: 海带, 复合酶, 海带多糖

Abstract: This study was designed to optimize the extraction of polysaccharides from Laminaria japonica by one-step hydrolysis with four different enzymes. By using orthogonal array design and analysis of variance based on one-factor-at-a-time experiments, the optimal hydrolysis conditions for polysaccharide extraction from Laminaria japonica were 0.5% cellulase, 1.0% pectinex, 1.0% papain, 1.0% xylanase, extraction temperature of 30 ℃, extraction time of 3 h and pH 5.0. Under these conditions, the extraction yield of polysaccharides was 78.9%, which was increased approximately 3-fold compared with that obtained by the traditional extraction method.

Key words: Laminaria japonica, multiple enzymes, polysaccharide

中图分类号:

TS201.1

翟为1，张美双1，张莉霞1，赵岩1,*，吴鹏2，张连学1,*. 复合酶法提取海带多糖工艺优化[J]. 食品科学, 2012, 33(18): 6-9.

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