嗜热链球菌和保加利亚乳杆菌混菌生物膜发酵特性及其抗逆性

食品科学 ›› 2013, Vol. 34 ›› Issue (3): 169-172.

嗜热链球菌和保加利亚乳杆菌混菌生物膜发酵特性及其抗逆性

玛依诺?木图拉¹,²,王坤¹,陈晓红¹,姜梅¹,李伟¹,董明盛³

1. 南京农业大学
2. 新疆师范大学
3. 南京农业大学食品科技学院

收稿日期:2012-02-24 修回日期:2013-01-11 出版日期:2013-02-15 发布日期:2017-12-29
通讯作者: 董明盛 E-mail:dongms@njau.edu.cn
基金资助:
国家“863”计划项目;教育部博士点基金（新教师类）;南京农业大学青年科技创新基金项目;江苏省自然科学基金项目

Fermentation Performance and Resistance of Mixed-species Biofilm Formed by Lactobacillus bulgaricus and Streptococcus thermophilus

Received:2012-02-24 Revised:2013-01-11 Online:2013-02-15 Published:2017-12-29
Supported by:
;Natural Science Foundation of Jiangsu province

摘要/Abstract

摘要： 摘要：利用扫描电子显微镜对嗜热链球菌(Streptococcus thermophilus)和保加利亚乳杆菌(Lactobacillus bulgaricus)在不锈钢网布及椰果粒表面形成的混菌生物膜进行观察，并对不锈钢网布表面混菌生物膜发酵特性及其抗逆性进行了初步研究，结果表明，Streptococcus thermophilus和Lactobacillus bulgaricus可在两种载体表面形成典型的混菌生物膜结构，且不锈钢网布表面混菌生物膜的发酵液菌体密度变化曲线与细菌S型生长曲线相似,但在停滞期和对数期不明显，发酵液pH呈现先下降后趋于平稳的趋势，终止pH值接近4.0；此外，不锈钢网布表面混菌生物膜比游离乳酸菌具有更强的抗性，并且可在15 d内稳定地释放较高浓度的游离乳酸菌。

Abstract: Abstract: In this study, the mixed-species biofilm on coconut and stainless steel mesh formed by Streptococcus thermophilus and Lactobacillus bulgaricus was observed using scanning electron microscope, then the preliminary study on fermentation performance and resistance of mixed-species biofilm on stainless steel mesh was conducted. The results showed that S. thermophilus and L. bulgaricus could form typical mixed-species biofilm on coconut and stainless steel mesh, and the bacterial density change curves of broth fermented by mixed-species boifilm on stainless steel mesh was similar with S-shaped growth curve，while the lag phase and logarithmic phase was not obvious; The pH value of broth fermented by mixed-species boifilm decreased in the early stages and then stabilized in the later stages, finally pH close to 4.0. In addition, the mixed-species boifilm has stronger resistance than free bacteria,and could release high concentrations of bacteria stably in 15 days.

中图分类号:

中图分类号：TS201.3

玛依诺?木图拉王坤陈晓红姜梅李伟董明盛. 嗜热链球菌和保加利亚乳杆菌混菌生物膜发酵特性及其抗逆性[J]. 食品科学, 2013, 34(3): 169-172.

参考文献

[1] 徐蓉蓉, 岳松龄, 胡涛. 生物膜中细菌的代谢特点及影响因素[J]. 国外医学.口腔医学分册, 2004, 31( 1): 42-43.
[2] COSTERTON J W, STEWART P S, GREENBERG E P. Bacterial Biofilms: A Common Cause of Persistent Infections[J]. Science, 1999,284 (5418): 1318-1322.
[3] 石磊.无处不在的生物膜[J]. 世界科学, 2005，11: 18-23.
[4] 干霞芳,李蒙英. 生物膜和生物膜形成菌的研究[J]. 安徽大学学报(自然科学版), 2007, 31(6): 91-94.
[5] 李彤,庄辉. 细菌生物膜的研究进展[J]. 中华微生物学和免疫学杂志, 2002, 22(3): 343-346.
[6] 张艾晓,陈君,朱雪娜等. 细菌生物膜及其在污染净化中的应用[J]. 科技通报,2009,25(1):103-108.
[7] 田超,司友斌,张圆圆.自然水体生物膜对Pb2+ Zn2+ 的吸附特性[J]. 农业环境科学学报,2011,30(6):
1180-1184.
[8] 张川. 光合细菌生物膜制氢反应器传输与产氢特性[D].重庆: 重庆大学, 2010.
[9] SPERANZA B, SINIGAGLIA M, CORBO M R. Non starter lactic acid bacteria biofilms: A means to control the growth of Listeria monocytogenes in soft cheese[J]. Food Control, 2009, 20(11): 1063-1067.
[10] RANGASWAMY V, RAMAKRISHNA S V. Lactic acid production by Lactobacillus delbrueckii in a dual reactor system using packed bed biofilm reactor[J]. Letters in Applied Microbiology, 2008, 46(6): 661-666.
[11] 周德庆. 微生物学实验教程[M]. 2版, 北京: 高等教育出版社, 2006:374.
[12] 李京宝, 韩峰, 于文功. 细菌生物膜研究技术[J]. 微生物学报, 2007, 47(3) : 558-561.
[13] 沈萍, 范秀荣, 李广武. 微生物学实验[M]. 3版, 北京: 高等教育出版社, 1999: 92-95.
[14] 贺银凤, 午日娜, 王建华. 乳酸菌的固定化方法及其发酵特性的研究[J]. 食品研究与开发,2006,27(5):16-18.