高效液相色谱法测定养殖水体中呋喃西林

食品科学 ›› 2013, Vol. 34 ›› Issue (2): 175-178.

高效液相色谱法测定养殖水体中呋喃西林

岑剑伟¹,李来好²,杨贤庆²,郝淑贤³,魏涯³,黄卉²,刁石强³,辛少平²

1. 中国水产科学研究院南海水产研究所
2. 中国水产科学研究院南海水产研究所
3. 南海水产研究所

收稿日期:2012-04-06 修回日期:2012-12-29 出版日期:2013-01-25 发布日期:2013-01-15
通讯作者: 岑剑伟 E-mail:251199510@qq.com
基金资助:
国家农业产业技术体系项目;国家农业科技成果转化资金项目;葡萄酒;农业部中央级公益性科研院所基本科研项目

Determination of nitrofurazone residue in aquaculture water by HPLC

Received:2012-04-06 Revised:2012-12-29 Online:2013-01-25 Published:2013-01-15

摘要/Abstract

摘要： 文章建立一套养殖水体中呋喃西林的高效液相色谱检测方法，通过呋喃西林理化性质研究为提取方法提供参考。试验结果表明液液提取不适用于水体中呋喃西林的提取，利用固相萃取效果最佳，比较了几种固相萃取小柱萃取效果，发现MCX、HLB、C18对NFZ具有保留作用，MCX适合用于处理偏碱性样液，HLB处理效果受pH影响较小，C18与HLB相比效果稍差。以HLB小柱浓缩水体中呋喃西林方法，回收率在85%～95%之间，相对标准偏差小于10%，检测限达到0.25μg/L，可应用于各类型养殖水体中的呋喃西林残留测定。

Abstract: We develop a method for nitrofurazone residue determined in the aquaculture water by High Performance Liquid Chromatography (HPLC).The stability of nitrofurazone was analyzed for the assistance to screen the determination condition. The results show that liquid-liquid extraction wasn’t suitable for extracting the nitrofurazone in water, while the solid-phase extraction is acceptable. The efficiency of several solid-phase extraction cartridges was compared to optimize the extract condition. MCX, HLB, C18 cartridge are capable of retaining nitrofurazone, which MCX is suitable for alkaline extract, HLB efficiency is insensitive to pH value, and the C18 is less effective than HLB. The methods, recovery of 85% -95%, relative standard deviation of less than10%, detection limit of 0.25μg/L, can be applied to scrutinize the nitrofurazone residue in aquaculture water.

中图分类号:

X824

岑剑伟李来好杨贤庆郝淑贤魏涯黄卉刁石强辛少平. 高效液相色谱法测定养殖水体中呋喃西林[J]. 食品科学, 2013, 34(2): 175-178.

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