关键词: 甜橙皮渣, 川皮苷, 超声波, 响应面分析

Abstract: The purpose of this study is to optimize operating conditions for ultrasonic-assisted extraction of nobiletin from sweet orange residues by response surface methodology. One-factor-at-a-time designs were used to examine the effects of four extraction parameters including ultrasonic power, ultrasonic treatment time, temperature and solid-to-solvent ratio on nobiletin yield. Further a mathematical model for nobiletin yield as a function of the extraction parameters was constructed by regression analysis based on a Box-Behnken design. The mathematical model was statistically significant and its determination coefficient (R2) was 0.9319. Moreover, the linear and quadratic terms had significant effects on nobiletin yield. Optimal extraction conditions were obtained by the use of absolute ethanol at a solid-to-solvent ratio of 1:20 g/mL and ultrasonic treatment for 45 min at 120 W. Under the optimal conditions, the experimental nobiletin yield was 209 μg/g and in good agreement with the predicted value (204 μg/g).

Key words: sweet orange residues, nobiletin, ultrasonic, response surface methodology (RSM)