响应面法优化新疆若羌大枣总黄酮提取工艺及抗氧化活性

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 123-126.

响应面法优化新疆若羌大枣总黄酮提取工艺及抗氧化活性

刘伟，南光明，李紫薇，腊萍，欧阳艳*

伊犁师范学院化学与生物科学学院

收稿日期:2012-06-29 修回日期:2012-10-19 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 欧阳艳 E-mail:oyy@ylsy.edu.cn
基金资助:
伊犁师范学院科研计划项目

Total Flavonoids from Xinjiang Ruoqiang Jujubes: Optimization of Extraction Conditions Using Response Surface Methodology and Antioxidant Activity

Received:2012-06-29 Revised:2012-10-19 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 在单因素试验基础上，以乙醇体积分数、提取温度、料液比为自变量，进行三因素三水平Box-Behnken试验设计，利用响应面分析法优化新疆若羌大枣总黄酮提取条件；将粗提物进一步分级萃取并对其抗氧化部位进行考察。结合实际可操作性得出大枣中总黄酮较优提取工艺为乙醇体积分数45%、提取温度90℃、料液比1:35(g/mL)、提取时间90min，总黄酮提取量为14.34mg山奈酚/g，达到预测值(14.647mg山奈酚/g)的97.9%。乙酸乙酯萃取物具有显著的抗氧化活性，与抗氧化剂BHT活性相当。

关键词: 新疆大枣, 响应面分析法, 总黄酮, 抗氧化活性

Abstract: This study was undertaken to optimize condition for the extraction of total flavonoids from Xinjiang Ruoqiang jujubes. One-factor-at-a-time method was used to investigate the effects of ethanol concentration, temperature, extraction time and solid-to-solvent ratio on the extraction yield of total flavonoids. Maximum extraction yield of total flavonoids was reached after 90 min of extraction. Using a three-variable, three-level Box-Behnken experimental design coupled with response surface methodology, ethanol concentration, temperature and solid-to-solvent ratio were optimized to be 45%, 90 ℃ and 1:35 (g/mL), respectively. Under the optimized conditions, the actual extraction yield of total flavonoids was 14.34 mg kaempferol equivalent/g, which was 97.9% as compared to the predicted value (14.647 mg kaempferol equivalent/g). The obtained extract had an ethyl acetate fraction that revealed potent antioxidant activity and was comparable to BHT.

Key words: Xinjiang Ruoqiang jujube, response surface methodology, total flavonoids, antioxidant activity

中图分类号:

R284.2

刘?伟，南光明，李紫薇，腊?萍，欧阳艳*. 响应面法优化新疆若羌大枣总黄酮提取工艺及抗氧化活性[J]. 食品科学, 2012, 33(22): 123-126.

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