黑灵芝多糖对阿霉素所致大鼠心肌损伤的保护作用

食品科学 ›› 2012, Vol. 33 ›› Issue (23): 283-286.

黑灵芝多糖对阿霉素所致大鼠心肌损伤的保护作用

李文娟，聂少平，邵灯寅，朱科学，李娜，郑温雅，谢明勇*

南昌大学食品科学与技术国家重点实验室

收稿日期:2012-07-01 修回日期:2012-11-12 出版日期:2012-12-15 发布日期:2012-12-12
通讯作者: 谢明勇 E-mail:xmync@sina.com
基金资助:
食物中多糖组分的高级结构表征及其活性功能作用机制;基于线粒体通透性转变孔道(mPTP)的黑灵芝多糖对高糖诱导血管内皮损伤的保护研究

Protective Effect of Polysaccharides from Ganoderma atrum against Adriamycin-Induced Myocardial Injury in Rats

Received:2012-07-01 Revised:2012-11-12 Online:2012-12-15 Published:2012-12-12

摘要/Abstract

摘要： 目的：探讨黑灵芝多糖对阿霉素(ADR)大鼠心肌损伤的保护作用。方法：腹腔注射ADR，建立心肌损伤模型，随机分为正常对照组、黑灵芝多糖高中低剂量组、ADR模型组，观察大鼠生理状况，检测乳酸脱氢酶(LDH)、肌酸激酶(CK)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性及线粒体膜电位、丙二醛(MDA)的含量。结果：与ADR模型组相比，黑灵芝多糖显著改善ADR引起体质量下降，提高CAT、GSH-Px、SOD活性和线粒体膜电位，降低LDH、CK活性和MDA含量。结论：黑灵芝多糖对ADR心肌损伤具有保护作用，其作用机制与降低心肌氧化应激、提高抗氧化防御、保护线粒体有关。

关键词: 黑灵芝多糖, 阿霉素, 心肌损伤, 氧化应激

Abstract: Objective: To investigate the protective effect of Ganoderma atrum polysaccharides against adriamycin (ADR)-induced cardiotoxicity in SD rats. Methods: A myocardial injury model induced by intraperitoneal injection of ADR was established in SD rats. SD rats were divided arbitrarily into five groups: control group, ADR model group, and three Ganoderma atrum polysaccharide treatment groups (administered at doses of 25, 50 mg/(kg·d) and100 mg/(kg·d) body weight, respectively for 7 consecutive days). ADR was administered on the 8th day at a dose of 20 mg/kg body weight. On the 14th day, the mice in each group were sacrificed and hearts were collected and determined for the activities of lactate dehydrogenase (LDH), creatine kinase (CK), catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), mitochondrial membrane potential and malondialdehyde (MDA) content. Moreover, mouse body weight and physiological conditions were examined. Results: Compared with ADR model group, Ganoderma atrum polysaccharides significantly attenuated ADR-induced body weight loss, increased CAT, SOD and GSH-Px activities and mitochondrial membrane potential, and decreased LDH and CK activities and MDA content in cardiac tissues. Conclusions: Ganoderma atrum polysaccharides can protect against ADR-induced myocardial injury in rats. The mechanism may be related to reducing oxidative stress, enhancing antioxidant defense and protecting mitochondria.

Key words: Ganoderma atrum polysaccharide, adriamycin, myocardial injury, oxidative stress

中图分类号:

Q949.32

李文娟，聂少平，邵灯寅，朱科学，李?娜，郑温雅，谢明勇*. 黑灵芝多糖对阿霉素所致大鼠心肌损伤的保护作用[J]. 食品科学, 2012, 33(23): 283-286.

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