食品科学 ›› 2012, Vol. 33 ›› Issue (24): 165-169.doi: 10.7506/spkx1002-6630-201224034

• 工艺技术 • 上一篇    下一篇

响应面法优化玉米醇溶蛋白提取工艺

李丽杰1,王英利1,赵一楠2   

  1. 1.内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特 010018;2.好丽友食品有限公司,北京 100015
  • 收稿日期:2012-09-05 修回日期:2012-12-03 出版日期:2012-12-25 发布日期:2012-12-12
  • 通讯作者: 李丽杰 E-mail:lilijie1978@163.com

Optimization of Zein Extraction from Corn Flour by Response Surface Methodology

LI Li-jie1,WANG Ying-li1,ZHAO Yi-nan2   

  1. 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;2. Orion Food Co. Ltd., Beijing 100015, China
  • Received:2012-09-05 Revised:2012-12-03 Online:2012-12-25 Published:2012-12-12
  • Contact: Li-Li-Jie E-mail:lilijie1978@163.com

摘要:

采用乙醇为提取剂,以玉米粉为原料提取玉米醇溶蛋白。探讨了液料比、浸提时间、乙醇体积分数、浸提温度对玉米醇溶蛋白提取率的影响。在单因素试验结果的基础上,确定各因素的分析水平,利用响应面中心组合法设计试验方案。以提取率为响应值,建立数学模型并得到玉米醇溶蛋白提取工艺优化组合为浸提温度55℃、浸提时间2.5h、液料比9:1(mL/g)、乙醇体积分数80%,并分析了双因素间的交互效应。

关键词: 玉米粉, 醇溶蛋白, 响应面法, 提取率, 交互效应

Abstract:

In this study, zein was extracted from corn flour using ethanol extraction. Response surface methodology was employed to establish optimum conditions for zein extraction. The effects of extraction time, extraction temperature, solid-to-liquid ratio and ethanol concentration on extraction efficiency were explored. As a result, a mathematical model was established. The optimum conditions for zein extraction were found to be extraction at 55 ℃ for 2.5 h using 80% ethanol at a solvent-to-solid ratio of 9:1 (mL/g). Moreover, we investigated the pairwise interactions of the various extraction conditions.

Key words: corn flour, zein, response surface methodology, extraction, interaction

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