菊苣酸下调3T3-L1前脂肪细胞中PGC-1α及FoxO4蛋白表达

doi:10.7506/spkx1002-6630-201321059

食品科学 ›› 2013, Vol. 34 ›› Issue (21): 297-301.doi: 10.7506/spkx1002-6630-201321059

菊苣酸下调3T3-L1前脂肪细胞中PGC-1α及FoxO4蛋白表达

肖海芳1,2，袁莉1，王静1，刘学波1,*

1.西北农林科技大学食品科学与工程学院，陕西杨凌 712100；
2.山东理工大学农业工程与食品科学学院，山东淄博 255049

收稿日期:2012-12-02 修回日期:2013-09-18 出版日期:2013-11-15 发布日期:2013-10-28
通讯作者: 刘学波 E-mail:xueboliu@yahoo.com.cn
基金资助:
国家自然科学基金项目(31000757)；“十二五”国家科技支撑计划项目(2012BAH30F03)

Downregulation of PGC-1α and FoxO4 Expressions by Chicoric Acid in 3T3-L1 Preadipocytes

XIAO Hai-fang1,2，YUAN Li1，WANG Jing1，LIU Xue-bo1,*

1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China；
2. School of Agricultural and Food Engineering, Shandong University of Technology, Zibo 255049, China

Received:2012-12-02 Revised:2013-09-18 Online:2013-11-15 Published:2013-10-28
Contact: LIU Xue-bo E-mail:xueboliu@yahoo.com.cn

摘要/Abstract

摘要：

通过菊苣酸作用于3T3-L1前脂肪细胞，检测其对PGC-1α及FoxO4蛋白表达的影响并探讨其作用机制。结果表明，菊苣酸能够有效抑制细胞中PGC-1α及FoxO4蛋白的表达；PI3K/Akt抑制剂LY294002可增强菊苣酸对两种蛋白的影响。提示菊苣酸通过PI3K/Akt信号通路下调PGC-1α及FoxO4蛋白的表达。

关键词: 菊苣酸；3T3-L1前脂肪细胞；PI3K/Akt；PGC-1&alpha, ；FoxO4

Abstract:

Chicoric acid, a naturally occurring polyphenolic compound, has been reported to have multiple bioactivities.
Our previous study indicated that chicoric acid induced the apoptosis of 3T3-L1 preadipocytes. PGC-1α and FoxO4 were
two important apoptotic proteins in preadipocytes. However, the impact and underlying mechanism of chicoric acid on
these proteins remain unknown. The purpose of this work was to investigate these issues. Our results revealed that chicoric
acid down-regulated the expressions of PGC-1α and FoxO4, and these effects were enhanced by the PI3K/Akt inhibitor
LY294002. These data suggested that the down-regulatory effect of chicoric acid on the expressions of PGC-1α and FoxO4
was achieved through PI3K/Akt signaling pathway.

Key words: chicoric acid；3T3-L1 preadipocytes；PI3K/Akt；PGC-1&alpha, ；FoxO4

中图分类号:

TS201.4

肖海芳1,2，袁莉1，王静1，刘学波1,*. 菊苣酸下调3T3-L1前脂肪细胞中PGC-1α及FoxO4蛋白表达[J]. 食品科学, 2013, 34(21): 297-301.

XIAO Hai-fang1,2，YUAN Li1，WANG Jing1，LIU Xue-bo1,*. Downregulation of PGC-1α and FoxO4 Expressions by Chicoric Acid in 3T3-L1 Preadipocytes[J]. FOOD SCIENCE, 2013, 34(21): 297-301.

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菊苣酸下调3T3-L1前脂肪细胞中PGC-1α及FoxO4蛋白表达

Downregulation of PGC-1α and FoxO4 Expressions by Chicoric Acid in 3T3-L1 Preadipocytes

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