食品科学

• 工艺技术 • 上一篇    下一篇

响应面法优化酸枣仁蛋白提取工艺

赵节昌   

  1. 徐州工程学院食品(生物)工程学院,江苏 徐州 221008
  • 出版日期:2013-08-25 发布日期:2013-09-03

Optimization of Protein Extraction from Zizyphi spinosi Semen by Response Surface Methodology

ZHAO Jie-chang   

  1. College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China
  • Online:2013-08-25 Published:2013-09-03

摘要:

为优化碱提酸沉法制备酸枣仁蛋白的工艺条件,通过单因素试验考察了液料比、pH值、浸提温度和浸提时间对酸枣仁蛋白提取率的影响,在单因素试验的基础上,采用Box-Behnken设计建立并分析各因素与提取率之间关系的数学模型。结果表明:碱提酸沉法制备酸枣仁蛋白的最佳工艺条件为液料比24:1(mL/g)、pH11、提取温度57℃、提取时间46min,在此条件下酸枣仁蛋白提取率的最大理论值为73.15%,实际蛋白提取率为73.11%,该回归模型具有较好的预测性能,可用于指导生产实践。

关键词: 酸枣仁, 蛋白, 碱提酸沉法, 响应面法

Abstract:

The extraction condition of protein from Zizyphi spinosi Semen by alkaline extraction and acid precipitation
was optimized by response surface methodology. The extraction efficiency of protein was investigated with respect to
four variables including solvent/solid ratio, pH, temperature and time. A mathematical model was fitted by means of Box-
Behnken design. The optimum conditions for extracting protein from Zizyphi spinosi Semen were determined as 24:1 (mL/g),
11, 57 ℃ and 46 min for solvent/solid ratio, pH, temperature and time, respectively. Under these conditions, the maximum
theoretical extraction yield of protein was 73.15%, which closely agreed with the actual value of 73.11%. Thus , the model
developed in this study has good prediction capability.

Key words: Zizyphi spinosi Semen, protein, alkaline extraction and acid precipitation method, response surface methodology