食品科学

• 基础研究 • 上一篇    下一篇

O/W型肉桂精油微乳液对灰葡萄孢的抑菌效果

何 艳1,应梦超1,王 韵2,孙 晔1,王一非1,*   

  1. 1.上海应用技术学院香料香精技术与工程学院,上海 201418;2.上海永安乳业有限公司,上海 201418
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 王一非
  • 基金资助:

    上海市教育委员会科研创新项目(12YZ165);上海应用技术学院引进人才科研启动项目(YJ2011-10)

Antimicrobial Activity of O/W Cinnamon Essential Oil Microemulsion against Botrytis cinerea

HE Yan1,YING Meng-chao1,WANG Yun2,SUN Ye1,WANG Yi-fei1,*   

  1. 1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China;
    2. Shanghai Yong’an Dairy Product Co. Ltd., Shanghai 201418, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: WANG Yi-fei

摘要:

利用微乳化技术,构建以肉桂精油为油相的食品级微乳体系,研究乙醇和丙酸作为助乳化剂对肉桂精油微乳体系相行为的影响,观察微乳液单独或结合壳聚糖对灰葡萄孢的抑菌效果。通过比较微乳区大小,选取配方为肉桂精油、乙醇、吐温-80质量比为1:3:6进行抑菌效果研究。在体外实验中,肉桂精油微乳液对灰葡萄孢的最低杀菌量(MFC)为250μL/L;在体内实验中,1g/L壳聚糖能提高500μL/L肉桂精油微乳液的抑菌效果,第4天时仍能完全抑制灰葡萄孢的生长,苹果灰霉病的防治率为100%。

关键词: 肉桂精油, 微乳液, 抑菌, 壳聚糖

Abstract:

In this work, a food-grade microemulsion system with cinnamon essential oil (CEO) was established, and the
effects of ethanol and propionic acid on the phase behavior of the microemulsion were explored. The antimicrobial activity
of the microemulsion alone or in combination with chitosan against Botrytis cinerea was also studied. The solubility of the
oil was improved in the presence of ethanol and propionic acid. The formulation of CEO, ethanol and Tween-80 in a mass
ratio of 1:3:6 was selected for antimicrobial assay. The minimum fungicidal concentration (MFC) of the microemulsion
against B. cinerea was 250 μL/L in vitro. The microemulsion at 500 μL/L, in combination with 1 g/L chitosan, showed a
complete inhibitory effect on gray mold in apples caused by B. cinerea after 4 days of storage.

Key words: cinnamon essential oil (CEO), microemulsion, antimicrobial activity, chitosan

中图分类号: