食品科学

• 基础研究 • 上一篇    下一篇

半干法酶解制备的魔芋葡甘低聚糖性质

邓利玲1,钟 耕1,2,3,*,刘倍毓1,何新春4   

  1. 1.西南大学食品科学学院,重庆 400716;2.西南大学 食品科学与工程国家级本科实验教学示范中心,重庆 400716;
    3.重庆市高校魔芋资源利用工程研究中心,重庆 400715;4.四川绵阳魔力科技有限公司,四川 绵阳 621000
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 钟 耕

Properties of Konjac Oligosaccharides Prepared by Semi-dry Enzymatic Hydrolysis

DENG Li-ling1,ZHONG Geng1,2,3,*,LIU Bei-yu1,HE Xin-chun4   

  1. 1. College of Food Science, Southwest University, Chongqing 400716, China;2. National Undergraduate Experimental Teaching
    Demonstration Center of Food Science and Engineering, Southwest University, Chongqing 400716, China;3. Konjac Resources
    Utilization Engineering Center of Chongqing Universities, Chongqing 400715, China;4. The Magic Technology Co. Ltd., in
    Mianyang, Mianyang 621000, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: ZHONG Geng

摘要:

以魔芋粉为原料,用半干法酶解制备魔芋葡甘低聚糖(KOS),并研究其结构特征、组成成分及理化性质。结果表明:半干法酶解制备的KOS呈白色粉状,白度可达90%以上,无味无臭,易溶于水,不溶于乙醇、乙醚等有机溶剂,比旋光度为左旋8.240°,比重为0.658g/mL,pH值在6.2左右,呈弱酸性。样品的干燥失重率较小,为0.705%。半干法酶解制备的KOS是一种吡喃型葡甘低聚糖,经液相色谱和飞行质谱联用(LC-TOF-TOF)分析,为甘露二糖到甘露十糖的混合物。

关键词: 半干法酶解, 魔芋葡甘低聚寡糖, 结构特征, 理化性质

Abstract:

Konjac flour was used as the raw material to prepare konjac oligosaccharides (KOS) by semi-dry enzymatic
hydrolysis, and then the structural characteristics, compositions and physicochemical properties of KOS were investigated. The
results showed that KOS was white, odorless and tasteless powder with whiteness up to 90%. It was highly soluble in water, but
insoluble in ethanol, ether and other organic solvents. The specific rotation of KOS was 8.240° degrees in a left-handed direction,
and the specific gravity was 0.658 g/mL, pH 6.2. The loss-weight rate was 0.705%. The KOS consisted of pyranose glucomannan
oligosaccharides as a mixture of mannan oligosaccharides (dimer through decamer) as analyzed by LC-TOF-TOF.

Key words: semi-dry enzymatic hydrolysis, konjac glucomannan oligosaccharides, structural characteristics, physicochemical properties

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