响应面法优化枯草芽孢杆菌产γ-聚谷氨酸发酵工艺

食品科学 ›› 0, Vol. ›› Issue (): 0-0.

• 生物工程 • 下一篇

响应面法优化枯草芽孢杆菌产γ-聚谷氨酸发酵工艺

贺杨扬¹,曾伟¹,王青龙²,王大芸¹,陈桂光²,梁智群²

1. 广西大学生命科学与技术学院
2. 广西大学生命科学与技术学院

收稿日期:2013-06-23 修回日期:2014-04-18 出版日期:2014-05-15 发布日期:2014-05-19
通讯作者: 梁智群 E-mail:zqliang@gxu.edu.cn
基金资助:
国家自然科学基金项目;广西研究生教育创新计划资助项目

Optimization of fermentation process on the Bacillus subtilis production of poly-γ-glutamic acid by response surface methodology

Received:2013-06-23 Revised:2014-04-18 Online:2014-05-15 Published:2014-05-19

摘要/Abstract

摘要： γ-聚谷氨酸是一种微生物源高分子多聚物，可广泛应用于工业、农业、食品、医药及化妆品等领域。以一株谷氨酸依赖型γ-聚谷氨酸产生菌Bacillus subtilis GXA-28为研究对象，利用响应面法系统优化其γ-聚谷氨酸发酵培养基成分。通用单因素优化实验、Plackett-Burman实验、最陡爬坡实验以及Box-Behnken实验构建响应方程，利用该方程预测得到最优培养基：蔗糖33.65 g/L、酵母膏0.4 g/L、NH4Cl 1.6 g/L、谷氨酸钠15 g/L、 KH2PO4 0.4 g/L、K2HPO4?3H2O 1.68 g/L、MgSO4?7 H2O 0.1 g/L、MnSO4?H2O 0.04 g/L。利用优化培养基，在40.2℃，160 r/min下摇瓶发酵22 h，γ-PGA产量达到16.63 g/L，比优化前提高20%，底物谷氨酸钠转化率达到100%。

关键词: γ-聚谷氨酸, Bacillus subtilis GXA-28, 响应面分析, 发酵培养基

Abstract: Poly-γ-glutamic acid, as a high molecular polymer produced by microorganism, is widely used in industry, agriculture, food, medicine and cosmetics. Systematic optimization of fermentation medium component on the production of poly-γ-glutamic acid using response surface methodology by a glutamic acid dependent strain of Bacillus subtilis GXA-28. Builded a response equation based on the single factor optimization experiment, Plackett-Burman experimental, steepest ascent experiment and Box-Behnken experimental, and predicted the optimal medium: sucrose 33.65 g/L, yeast extract 0.4 g/L, NH4Cl 1.6 g/L, monosodium glutamate 15 g/L, KH2PO4 0.4 g/L、K2HPO4?3H2O 1.68 g/L、MgSO4?7 H2O 0.1 g/L、MnSO4?H2O 0.04 g/L. The yield of poly-γ-glutamic acid reach 16.63 g/L which increased by 20% than ever before and the conversion rate of substrate glutamate reach 100% by using the optimized medium under 40.2 oC and 160 r/min for 22 h.

Key words: Poly-γ-glutamic acid, Bacillus subtilis GXA-28, response surface analysis, fermentation medium

中图分类号:

贺杨扬曾伟王青龙王大芸陈桂光梁智群. 响应面法优化枯草芽孢杆菌产γ-聚谷氨酸发酵工艺[J]. 食品科学, 0, (): 0-0.

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响应面法优化枯草芽孢杆菌产γ-聚谷氨酸发酵工艺

Optimization of fermentation process on the Bacillus subtilis production of poly-γ-glutamic acid by response surface methodology

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