食品科学

• 工艺技术 • 上一篇    下一篇

超声波协同酶法提取香椿老叶可溶性膳食纤维的工艺优化

刘 静,李湘利,孔 娴,梁宝东,胡彦营   

  1. 济宁学院生命科学与工程系,山东 曲阜 273155
  • 出版日期:2014-06-25 发布日期:2014-07-03
  • 通讯作者: 刘 静 E-mail:liujingpretty@163.com
  • 基金资助:

    山东省高等学校科技计划项目(J12LF52)

Optimization of Ultrasonic-Assisted Enzymatic Hydrolysis for the Extraction of Soluble Dietary Fiber fromOld Leaves of Toona sinensis

LIU Jing, LI Xiang-li, KONG Xian, LIANG Bao-dong, HU Yan-ying   

  1. Department of Life Science and Engineering, Jining University, Qufu 273155, China
  • Online:2014-06-25 Published:2014-07-03
  • Contact: LIU Jing E-mail:liujingpretty@163.com

摘要:

以香椿老叶为试材,研究超声波协同酶法提取香椿老叶可溶性膳食纤维的影响因素,通过响应面优化试验确定最佳提取工艺参数。结果表明:影响香椿老叶可溶性膳食纤维提取率的因素主次顺序为超声时间>纤维素酶添加量>pH值>超声温度;最佳提取条件为纤维素酶添加量0.50%、超声温度60.0 ℃、超声时间53.0 min、pH 6.0;此条件下可溶性膳食纤维的提取率可达7.11%,产品杂质含量低,持水力和膨胀力分别为7.29 g/g和4.40 mL/g。

关键词: 香椿, 可溶性膳食纤维, 纤维素酶, 超声波, 响应面法

Abstract:

The ultrasonic-assisted enzymatic extraction of soluble dietary fiber (SDF) from old leaves of Toona sinensis was
optimized using response surface methodology. Extraction efficiency was affected in decreasing order by ultrasonication
time, cellulase dosage, pH and temperature, and the optimum extraction conditions for these four parameters were 53.0 min,
0.50%, 6.0 and 60.0 ℃, respectively. Under the optimized conditions, the extraction yield of SDF was 7.11% with low levels
of impurities. The water holding capacity and expansibility of SDF products were 7.29 g/g and 4.40 mL/g, respectively.

Key words: Toona sinensis, soluble dietary fiber, cellulase, ultrasonic, response surface methodology

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