食品科学

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响应面法优化双酶水解技术生产婴幼儿配方米粉工艺

刘梅森,林汉卿,张向阳,高丽霄,何小勇,张织芬,臧成国   

  1. 深圳市味奇生物科技有限公司,广东 深圳 518109
  • 出版日期:2014-11-25 发布日期:2014-12-09
  • 基金资助:

    广东省产业技术研究与开发资金项目(2012B020402002)

Optimization of Double Enzymatic Hydrolysis of Rice for Production of Infant Formula Rice Powder Using Response Surface Methodology

LIU Mei-sen, LIN Han-qing, ZHANG Xiang-yang, GAO Li-xiao, HE Xiao-yong, ZHANG Zhi-fen, ZANG Cheng-guo   

  1. Shenzhen Weicky Biotechnology Co. Ltd., Shenzhen 518109, China
  • Online:2014-11-25 Published:2014-12-09

摘要:

为优化双酶水解技术生产婴幼儿米粉工艺,以α-淀粉酶添加量、β-淀粉酶添加量、调浆水温度为主要影响因素,结合实际生产中的其他水解条件,在单因素试验基础上,运用Box-Behnken试验设计原理,探讨α-淀粉酶添加量、β-淀粉酶添加量、调浆水温度的最佳组合。结果表明:α-淀粉酶添加量0.04‰(相当于0.15 U/g)、β-淀粉酶添加量0.26%(相当于1 820 U/g)、调浆水温度70.2 ℃时生产米粉的淀粉消化指数高达39.26%,与市售品牌米粉相比,淀粉消化指数提高10%以上。

关键词: 婴幼儿配方米粉, &alpha, -淀粉酶, &beta, -淀粉酶, 调浆水温度, 消化指数, 响应面法

Abstract:

The enzymatic hydrolysis of rice slurry with α-amylase and β-amylase for use in infant formula rice powder
was optimized using a Box-Behnken design with response surface methodology. The optimal hydrolysis conditions
were established as follows: 0.04‰ (equivalent to 0.15 U/g) α-amylase, 0.26% (equivalent to 1 820 U/g) β-amylase, and
hydrolysis at 70.2 ℃, yielding a starch digestion index as high as 39.26%, which represented an over 10% increase over that
reported for commercial infant rice powder.

Key words: infant formula rice powder, α-amylase, β-amylase, mashing temperature, starch digestion index, response surface methodology

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