牛乳制品中青霉素酶Alphalisa的检测方法

doi:10.7506/spkx1002-6630-201514035

食品科学

牛乳制品中青霉素酶Alphalisa的检测方法

张姜琳1,2，聂福平1，杨俊1，李应国1，肖进文1，蔡家利2，袁增壮3，叶自霞3，王国民1，王昱1,*

1.重庆出入境检验检疫局技术中心，重庆 400020；
2.重庆理工大学药学与生物工程学院，重庆 400054；3.西南大学动物科技学院，重庆 400716

出版日期:2015-07-25 发布日期:2015-07-15
通讯作者: 王昱
基金资助:
重庆市科技攻关项目（2014yykfA80017；2010GGC239）；国家质检总局质检项目（2012IK029）

Establishment of the Alphalisa Method for the Detection of Penicillinase in Milk Products

ZHANG Jianglin1,2, NIE Fuping1, YANG Jun1, LI Yingguo1, XIAO Jinwen1, CAI Jiali2, YUAN Zengzhuang3, YE Zixia3, WANG Guomin1, WANG Yu1,*

1. Technical Center, Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing 400020, China;
2. Institute of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China;
3. College of Animal Science and Technology, Southwest University, Chongqing 400716, China

Online:2015-07-25 Published:2015-07-15
Contact: WANG Yu

摘要/Abstract

摘要：

建立牛乳制品中青霉素酶Alphalisa的检测方法。将青霉素酶进行生物素化，与样品中的青霉素酶竞争结合鼠源性青霉素酶单克隆抗体，利用Alphalisa反向竞争的反应模式，对牛乳中的青霉素酶实施检测。建立的Alphalisa检测方法能特异性识别样品中存在的青霉素酶，方法的线性范围在2.5～500 IU/mL；检出限和定量限分别为1.6 IU/mL和5 IU/mL；批间批内变异度均小于10%。结果表明，该方法能够替代传统酶联免疫吸附实验方法，成为青霉素酶的实验室筛查或检测方法。

关键词: 青霉素酶, Alphalisa检测, 牛乳制品

Abstract:

Penicillinase is one of the rick drugs present in milk products. In this article, the standard penicillinase was
biotinylated, and competitively bound to mouse anti-penicillinase monoclonal antibodies by reverse competitive Alphalisa.
The established Alphalisa detection method was shown to specifically identify penicillinase present in the samples. The
variations between batches were less than 10%, with the limit of detection and the limit of quantitation of 1.6 IU/mL and
5 IU/mL, respectively, and the linearity range of 2.5-500 IU/mL. The results show that this method can replace the
traditional ELISA method as a laboratory screening or detection method for penicillinase.

Key words: penicillinase, alphalisa detection, milk products

中图分类号:

R155.5

张姜琳1,2，聂福平1，杨俊1，李应国1，肖进文1，蔡家利2，袁增壮3，叶自霞3，王国民1，王昱1,*. 牛乳制品中青霉素酶Alphalisa的检测方法[J]. 食品科学, doi: 10.7506/spkx1002-6630-201514035.

ZHANG Jianglin1,2, NIE Fuping1, YANG Jun1, LI Yingguo1, XIAO Jinwen1, CAI Jiali2, YUAN Zengzhuang3, YE Zixia3, WANG Guomin1, WANG Yu1,*. Establishment of the Alphalisa Method for the Detection of Penicillinase in Milk Products[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201514035.

牛乳制品中青霉素酶Alphalisa的检测方法

Establishment of the Alphalisa Method for the Detection of Penicillinase in Milk Products

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