食品科学

• 生物工程 • 上一篇    下一篇

鲤鱼中腐败希瓦氏菌生长模型的建立和验证

周 康,杨兆锞,刘书亮,韩新锋   

  1. 四川农业大学食品学院,四川 雅安 625014
  • 出版日期:2015-08-15 发布日期:2015-08-17

Modeling and Validation of the Growth of Shewanella putrefaciens from Cyprinus carpio

ZHOU Kang, YANG Zhaoke, LIU Shuliang, HAN Xinfeng   

  1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2015-08-15 Published:2015-08-17

摘要:

利用市售鲤鱼中分离鉴定得到的腐败希瓦氏菌进行了生长动力学模型的建立。通过预测微生物学的方法比较Logistic、Gompertz和Baranyi模型的生物学特性和应用特性,对3 种模型所得参数进而构建了二级模型并分别进行了内部和外部验证。结果表明:Logistic、Gompertz和Baranyi模型在内部验证中的r2分别为0.982 4、0.983 6和0.988 4,偏差因子(bias factor,Bf)分别为0.985 0、0.995 0和0.970 0;而外部验证Bf值分别为0.804、1.177和0.826。Baranyi模型在对微生物生长的预测性上不仅准确,而且能够更加有效地进行食品安全预警,在实际的农产品和食品领域具有很好的实用价值。

关键词: 生长动力学模型, 内部验证, 外部验证, 腐败希瓦氏菌

Abstract:

A strain isolated from marketed common carp (Cyprinus carpio), identified as Shewanella putrefacens by
biological and molecular identification methods, was used in the growth modeling study. The obtained data were fitted with
three different primary models including Logistic, Gompertz and Banrayi models and the fitted models were validated with
coefficients of determination (r2) of 0.982 4, 0.983 6 and 0.988 4, as well as bias factor (Bf) of 0.985 0, 0.995 0 and 0.970 0
in the internal validation and 0.804, 1.177 and 0.826 in the external validation, respectively. The results showed that the
Baranyi model not only had a goodness-of-fit, but also could provide effective early warnings of microbial food safety risks.
Therefore, the Baranyi model has a great potential for application in agricultural products and foods.

Key words: growth kinetic model, internal validation, external validation, Shewanella putrefacens

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