四棱豆总黄酮抗氧化和抗肝损伤作用研究

doi:10.7506/spkx1002-6630-201515038

摘要/Abstract

摘要：

目的：探讨四棱豆总黄酮体外和体内抗氧化能力以及对CCl4诱导小鼠肝损伤的修复效果。方法：利用超声波辅助提取四棱豆种子总黄酮，以1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基、2,2’-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（2,2’-azinobis-（3-ethylbenzothiazoline-6-sulphonate），ABTS）自由基、羟自由基的清除率和还原力表征四棱豆总黄酮的抗氧化活性。利用CCl4诱导昆明小鼠肝损伤，以不同剂量的四棱豆总黄酮灌胃7 d，测定血清中谷草转氨酶、碱性磷酸酶、谷丙转氨酶、γ-谷氨酰转肽酶活性和总胆红素含量，测定肝、肾组织中的超氧化物歧化酶、过氧化氢酶活性及谷胱甘肽、丙二醛含量，免疫组化法观察肝细胞中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）表达水平，苏木精-伊红染色观察肝脏组织病理学变化。结果：体外抗氧化实验表明四棱豆总黄酮具有较强的抗氧化效果，四棱豆总黄酮各剂量组均能降低CCl4致小鼠急性肝损伤血清谷草转氨酶、碱性磷酸酶、谷丙转氨酶、谷氨酰转肽酶活性和总胆红素含量升高，降低肝、肾组织中丙二醛含量，增强超氧化物歧化酶、过氧化氢酶活性和谷胱甘肽含量，抑制肝细胞中TNF-α表达，减轻CCl4对肝组织的损伤。结论：四棱豆总黄酮对CCl4致小鼠急性肝损伤具有一定的保护作用，其保护机制可能与清除自由基、抑制脂质过氧化、抑制TNF-α表达有关。

关键词: 四棱豆, 抗氧化活性, 护肝活性, 肿瘤坏死因子-&alpha

Abstract:

Objective: To investigate the hepatoprotective and antioxidant effects of total flavonoids from Psophocarpus
tetragonolobus (L.) DC. seeds (TFP) in vitro and in vivo. Methods: The antioxidant activity of TFP in vitro was determined
by scavenging capacities against DPPH, ABTS, and hydroxyl free radicals as well as reducing power. Mice with carbon
tetrachloride (CCl4)-induced liver injury were used to assess the hepatoprotective and antioxidant activity of TFP in vivo.
The activities of alkaline phosphatase (ALP), glutamate pyruvate transaminase (ALT), aspartate aminotransferase (AST) and
gamma-glutamyl transpeptidase (GGT) and total bilirubin (TB) level in serum, and the levels of malondialdehyde (MDA)
and glutathione (GSH) and the activities of superoxide dismutase (SOD) and catalase (CAT) in the liver and kidney of mice
were assayed using standard procedures. Immunohistochemistry was used to detect the expression of TNF-α in hepatic
tissue. Meanwhile hepatic histopathological examination was observed. Results: TFP exhibited strong antioxidant activity.
TFP at all investigated doses significantly lowered the levels of serum ALT, AST, ALP, GGT, TC, TG and TB and the
levels of hepatic and renal MDA, enhanced the activities of SOD and CAT, and greatly inhibited the expression of TNF-α in
mice with acute liver injury induced by CCl4, thus ameliorating the liver damage in mice. Conclusion: TFP has a significant
hepatoprotective effect on CCl4-induced acute liver injury in mice, possibly by scavenging free radicals and inhibiting lipid
peroxidation and TNF-α expression.

Key words: Psophocarpus tetragonolobus (L.) DC., antioxidant activity, hepatoprotective activity, tumor necrosis factor-&alpha, (TNF-α)

中图分类号:

TS201.4

黄小波，付明，陈东明. 四棱豆总黄酮抗氧化和抗肝损伤作用研究[J]. 食品科学, doi: 10.7506/spkx1002-6630-201515038.

HUANG Xiaobo, FU Ming, CHEN Dongming. Antioxidant Activity and Hepatoprotective Activity of Total Flavonoids from Psophocarpus tetragonolobus (L.) DC. Seeds[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201515038.

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四棱豆总黄酮抗氧化和抗肝损伤作用研究

Antioxidant Activity and Hepatoprotective Activity of Total Flavonoids from Psophocarpus tetragonolobus (L.) DC. Seeds

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