浸提法、超声波法和微波法提取紫薯花色苷的抗氧化性比较研究

doi:10.7506/spkx1002-6630-201519015

食品科学

浸提法、超声波法和微波法提取紫薯花色苷的抗氧化性比较研究

1.沈阳农业大学食品学院，辽宁沈阳 110866；2.渤海大学食品科学研究院，辽宁省食品安全重点实验室，
辽宁锦州 121013；3.辽宁医学院食品科学与工程学院，辽宁锦州 121001

出版日期:2015-10-15 发布日期:2015-10-20

Comparative Antioxidant Activities of Anthocyanins Extracted from Purple Sweet Potato by Organic Solvent,Ultrasonic-Assisted and Microwave-Assisted Extraction

1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
2. Food Safety Key Laboratory of Liaoning Provinve, Food Science Research Institute, Bohai University, Jinzhou 121013, China;
3. College of Food Science and Engineering, Liaoning Medical University, Jinzhou 121001, China

Online:2015-10-15 Published:2015-10-20

摘要/Abstract

摘要：

本实验通过羟自由基（·OH）清除率、1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基清除率、还原力、超氧阴离子自由基（O2－·）清除率、总抗氧化性和金属螯合能力6 个抗氧化测定方法对浸提法、微波辅助提取法和超声波辅助提取法提取的紫薯花色苷的抗氧化活性进行了综合评价。结果表明：除金属螯合能力较弱外，其余5 种抗氧化活性检测结果均表明紫薯花色苷具有一定的抗氧化能力，且5 种抗氧化活性检测结果一致性地表明微波辅助提取法、超声波辅助提取法和浸提法提取的紫薯花色苷抗氧化能力依次降低，它们都总体表现为对·OH、DPPH自由基、O2－·的清除能力较强，还原力和总抗氧化性次之，金属螯合能力最差；采用超声波或微波辅助提取有助于保持提取的紫薯花色苷的抗氧化活性，且微波辅助提取法效果最好；对紫薯花色苷抗氧化活性的检测和判定可以·OH、DPPH自由基和O2－·清除能力作为主要指标。

关键词: 紫薯花色苷, 抗氧化性, 对比

Abstract:

The antioxidant activity of anthocyanins extracted from purple sweet potato by organic solvent, ultrasonicassisted
or microwave-assisted extraction was comparatively evaluated by radical scavenging activities against hydroxyl,
DPPH and superoxide anion radicals, total antioxidant capacity (TAC), reducing power and ferrous ion chelating activity.
The results showed that the anthocyanins extracted from purple sweet potato, irrespective of the extraction method used,
exerted antioxidant activities except for ferrous ion chelation. Moreover, the results from 5 antioxidant assays consistently
indicated that the antioxidant capacity of anthocyanins extracted by microwave-assisted, ultrasonic-assisted and organic
solvent extraction revealed a decreasing trend in turn. The extracted anthocyanins showed the strongest ability to scavenge
free radicals followed by reducing power and total antioxidant capacity, and possessed the lowest ferrous ion-chelating
activity. Both ultrasonic-assisted and microwave-assisted extraction are beneficial for maintaining antioxidant activity of
anthocyanins extracted from purple sweet potato, with the latter being superior to the former.

Key words: anthocyanins from purple sweet potato, antioxidant activity, comparison

中图分类号:

TS20

朱璐，董福，冯叙桥，王晶晶，杜玉慧，程丞. 浸提法、超声波法和微波法提取紫薯花色苷的抗氧化性比较研究[J]. 食品科学, doi: 10.7506/spkx1002-6630-201519015.

ZHU Lu, DONG Fu, FENG Xuqiao, WANG Jingjing, DU Yuhui, CHENG Cheng. Comparative Antioxidant Activities of Anthocyanins Extracted from Purple Sweet Potato by Organic Solvent,Ultrasonic-Assisted and Microwave-Assisted Extraction[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201519015.

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浸提法、超声波法和微波法提取紫薯花色苷的抗氧化性比较研究

Comparative Antioxidant Activities of Anthocyanins Extracted from Purple Sweet Potato by Organic Solvent,Ultrasonic-Assisted and Microwave-Assisted Extraction

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