丰年虫卵壳色素的制备及稳定性与体外抗氧化活性研究

doi:10.7506/spkx1002-6630-201605018

摘要/Abstract

摘要：

利用超声波辅助乙醇溶液提取丰年虫卵壳色素，采用红外光谱对色素结构进行初步鉴定，通过紫外-可见扫描光谱分别研究光照、温度、pH值、食品添加剂、金属离子及氧化还原剂对色素稳定性的影响，分别测定色素的总抗氧化能力、羟自由基（·OH）与2,2’-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐自由基（2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) free radical，ABTS＋·）清除活性，以考察色素体外抗氧化活性。结果表明：丰年虫卵壳色素为黑褐色粉末，可能由一类含有羟基、烷烃—CH2、碳碳三键及醚键的酰胺-芳香族复合物组成，其提取液在397 nm波长处获得最大吸收峰。色素对光和高温敏感，pH值变化、蔗糖、碳酸钠、碳酸氢钠、柠檬酸钠、Na＋及K＋对色素稳定性影响较小，苯甲酸钠、Ca2＋、Zn2＋、Fe3＋、Fe2＋、Cu2＋及Pb2＋则对色素均具有一定程度的破坏，酸性条件、柠檬酸、酒石酸、可溶性淀粉、Mg2＋及Al3＋则对色素具有增色效应。丰年虫卵壳色素容易被氧化剂破坏，但具有较高的耐还原性和良好的体外抗氧化活性，清除·OH与ABTS＋·的IC50分别为6.104、0.377 mg/mL。说明该色素可作为天然添加剂应用于食品中，起到一定的抗氧化作用。

关键词: 丰年虫卵壳, 色素, 稳定性, 体外抗氧化

Abstract:

Chirocephalus diaphanous eggshell was ground, defatted and sieved before being subjected to ultrasonic-assisted
extraction with 60% ethanol solution as the solvent to obtain pigment. The structure of the pigment as well as its maximum
absorption peak was determined by infrared and ultraviolet-visible spectroscopy. Then effects of light, temperature, pH,
food additives, metal ions, oxidant and reductant on its stability were investigated. Total antioxidant capacity and hydroxyl
and ABTS radical scavenging activities of the pigment were measured by in vitro. The results showed that the pigment
was a dark brown powder composed of amide-aromatic compounds with –OH, –CH2–, –C≡C– and –C–O–C–, and the
maximum absorption peak appeared at 397 nm. The pigment was sensitive to outdoor natural light, ultraviolet light and
high temperature. pH, sacrose, sodium carbonate, sodium bicarbonate, sodium citrate, Na+ and K+ could affect the stability
of the pigment. Acidic environment, citric acid, tartaric acid, soluble starch, Mg2+ and Al3+ had a hyperchromic effect on the
pigment. However, sodium benzoate, Ca2+, Zn2+, Fe3+, Fe2+, Cu2+ and Pb2+ could destroy the pigment in different degrees. The
pigment could be easily damaged by oxidant, but it had high capacity to resist reduction. The pigment possessed favorable
total antioxidant activity, and its IC50 for scavenging hydroxyl and ABTS radicals were 6.104 and 0.377 mg/mL, respectively.
Therefore, the pigment can be used as a natural antioxidant.

Key words: Chirocephalus diaphanous eggshell, pigment, stability, in vitro antioxidant activity

中图分类号:

TS202

许彦腾，张建新，何秋芬，文欢. 丰年虫卵壳色素的制备及稳定性与体外抗氧化活性研究[J]. 食品科学, doi: 10.7506/spkx1002-6630-201605018.

XU Yanteng, ZHANG Jianxin, HE Qiufen, WEN Huan. Preparation, Stability and in Vitro Antioxidant Activity of Pigment from Chirocephalus diaphanous Eggshell[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201605018.

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丰年虫卵壳色素的制备及稳定性与体外抗氧化活性研究

Preparation, Stability and in Vitro Antioxidant Activity of Pigment from Chirocephalus diaphanous Eggshell

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