虾壳中复合钙粉的制备工艺优化及大鼠对其吸收效果的评价

doi:10.7506/spkx1002-6630-201614001

摘要/Abstract

摘要：

为研究虾壳中复合钙粉的制取工艺及评价大鼠对其吸收效果，以南美白对虾虾壳为研究对象，采用胰蛋白酶-柠檬酸法制取钙粉，进而以缺钙为模型研究大鼠对复合钙粉的吸收效果。结果表明：采用胰蛋白酶-柠檬酸制取复合钙粉的最佳工艺为加酶量2.5×103 U/g、酶解pH 8.0、酶解时间1.5 h、酶解温度45 ℃，此时，蛋白水解度为57.24%；柠檬酸浓度0.35 mol/L、柠檬酸用量20 mL、处理时间1.5 h时，钙溶出率为85.78%；制取的复合钙粉中钙含量为19.6%，总氨基酸含量为18.3%。复合钙粉质地细腻，呈淡褐色，无异味。动物实验表明，从虾壳中制取的复合钙粉的大鼠钙吸收效果显著（P＜0.05），灌胃剂量66.5 mg/（kg•d）（以钙含量及大鼠体质量计）的复合钙粉能明显提高大鼠血清钙、磷水平，促进大鼠正常生长。胰蛋白酶结合柠檬酸能有效地从虾壳中制取复合钙粉，其吸收效果优于相同钙含量的碳酸钙。

关键词: 南美白对虾虾壳, 复合钙粉, 胰蛋白酶, 柠檬酸

Abstract:

Purpose: To optimize the preparation of blended calcium powders from shrimp shell and to evaluate its calcium
absorption in rats. Methods: The extraction of blended calcium powders from shrimp shell (Penaeus vannamei) by the
combined use of trypsin and citric acid was investigated. Furthermore, the effects of calcium powder on growth, calcium
absorption in rats was studied by establishing calcium deficiency model. Results: The optimized preparation conditions
were determined as follows: initial hydrolysis with 2.5 × 103 U/g of trypsin at pH 8.0 and 45 ℃ for 1.5 h followed by
treatment with 20 mL of 0.35 mol/L citric acid for 1.5 h. Under these conditions, the rate of proteolysis was 57.24%, and the
dissolution rate of calcium was 85.78%. Furthermore, the calcium power contained 19.6% calcium and 18.3% total amino
acids, which was a hazel powder with a delicate texture and without off-flavor. Animal experiments showed that the calcium
powder prepared from shrimp shell significantly improved calcium absorption in rats (P < 0.05), and that gavage dose of
66.5 mg/(kg·d) (calculate based on calcium content and the body weight of rats) could improve the contents of calcium and
phosphorous in serum. Conclusion: Trypsin combined with citric acid offers a mild and effective way to extract blended
calcium powder from shrimp shell, which exhibits better absorption than of CaCO3 with the same calcium content.

Key words: shrimp shell, blended calcium powders, trypsin, citric acid.

中图分类号:

TS254.9

张文静，陈舜胜. 虾壳中复合钙粉的制备工艺优化及大鼠对其吸收效果的评价[J]. 食品科学, doi: 10.7506/spkx1002-6630-201614001.

ZHANG Wenjing, CHEN Shunsheng. Preparation of Blended Calcium Powders from Shrimp Shell and Evaluation of Its Calcium Absorption in Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614001.

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