食品科学

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新疆传统发酵酸驼乳优势菌种短乳杆菌高密度培养技术

沙吉坦穆·克依斯尔,古丽皮艳·托乎提,努丽艳·阿不都米力克,日孜万古力·艾山,努尔古丽·热合曼*   

  1. 新疆师范大学生命科学学院,新疆 乌鲁木齐 830054
  • 出版日期:2016-12-15 发布日期:2016-12-21

High-Density Cultivation of Lactobacillus brevis as a Dominant Strain Isolated from Xinjiang Traditional Fermented Camel Milk

KAYSER Sajidam, TOHTI Gulpiya, ABDUMILIK Nurya, HASAN Rezwangul, RAHMAN Nurgul*   

  1. School of Life Sciences, Xinjiang Normal University, Ürümqi 830054, China
  • Online:2016-12-15 Published:2016-12-21

摘要: 以分离自传统发酵酸驼乳中的优势菌种短乳杆菌作为材料,通过单因素试验,确定微囊化短乳杆菌的最佳碳源为蔗糖,最适质量浓度为15 g/L;最佳氮源为胰蛋白胨,最适质量浓度为10 g/L;最佳促生长因子为玉米浆,最适体积分数15%;最佳酸碱缓冲剂为CaCO3,最适质量浓度为5 g/L。 在此基础上,采用响应曲面法分析了4 个因子的交互作用及其最佳水平范围,高密度发酵培养的培养基优化配方为:乳清粉60 g/L、蔗糖18 g/L、胰蛋白胨13.02 g/L、玉米浆130 mL/L、CaCO3 3.29 g/L。

关键词: 传统发酵酸驼乳, 短乳杆菌, 高密度培养, 响应曲面法

Abstract: In this study, the one-factor-at-a-time method was employed to determine that the optimum carbon source for
liquid-core microcapsules of Lactobacillus brevis M3-3 as a dominant strain isolated from Xinjiang traditional fermented
camel milk was sucrose at 15 g/L, the optimum nitrogen source was typtone at 10 g/L, the optimum growth promoting
factor was corn steep liquor at 15% and the optimum pH buffering agent was CaCO3 at 5 g/L. The interaction among the
four factors was studied using response surface methodology to determine their optimum levels. The optimum medium
formulation for high-density cultivation of the strain was found to be composed of 60 g/L whey powder, 18 g/L sucrose,
13.02 g/L typtone, 130 mL/L corn steep liquor, and 3.29 g/L CaCO3.

Key words: traditional fermented camel milk, Lactobacillus brevis, high-density cultivation, response surface methodology

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