关键词: 生物传感器, 人参皂苷, 固定化, 小肠

Abstract: Using sodium alginate-starch gel as a fixing agent, small intestine tissues from rats were fixed between two
nuclear microporous membranes to make a sandwich-type membrane, which was then fixed onto a glassy carbon electrode to
produce a biosensor electrode. The electrochemical workstation could test the current generated when ginsenoside stimulated
the corresponding receptor. The results showed that in a low concentration range, the electric current value grew linearly,
suggesting that the amount of receptor was greater than the amount of ligands. However, the final part of the growth curve
exhibited a non-linear increase, suggesting that the receptors were gradually saturated. The action of ginsenoside and its
receptor was fitted to a hyperbolic curve with Origin 9 software (R2 Adj= 0.994), which indicated the presence of ginsenoside
receptors in the small intestine. The constant for ginsenoside and its receptor obtained with the double reciprocal method
(R2Adj= 0.995) was 2.969 × 10-16 g/mL. This study has not only quantitatively determined the interaction of ginsenoside and its
receptor substances with a new biosensor, but also has provided a simple approach for evaluating health benefits of ginseng
saponins from the perspective of intestinal receptors and investigating the mechanism of ligand-receptor interaction.

Key words: biological biosensor, ginsenoside, immobilization, small intestine