铁观音多酚提取物对小鼠肝Cyp450s的影响

doi:10.7506/spkx1002-6630-201707032

食品科学

铁观音多酚提取物对小鼠肝Cyp450s的影响

任红1,2，刘冬2，郑少杰1，严弋敬2，孙海燕2,*，明建1,*

1.西南大学食品科学学院，重庆 400715；2.深圳职业技术学院深圳市发酵精制检测系统重点实验室，广东深圳 518055

出版日期:2017-04-15 发布日期:2017-04-24

Effect of Polyphenols Extracted from Tieguanyin Tea on the mRNA and Protein Expression Levels of Hepatic Cytochromes P450 (Cyp450s) in Mice

REN Hong1,2, LIU Dong2, ZHENG Shaojie1, YAN Yijing2, SUN Haiyan2,*, MING Jian1,*

1. College of Food Science, Southwest University, Chongqing 400715, China；2. Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen 518055, China

Online:2017-04-15 Published:2017-04-24

摘要/Abstract

摘要： 为探究铁观音多酚提取物对小鼠肝Cyp450s的影响，采用实时荧光定量反转录聚合酶链式反应法和Westernblot法评价铁观音多酚提取物对小鼠肝Cyp3a11、Cyp2d22、Cyp2c37 3 种酶的mRNA和蛋白表达。结果显示，与空白对照组相比，低剂量铁观音多酚提取物（150 mg/（kg·d））能显著诱导Cyp3a11、Cyp2d22、Cyp2c37的mRNA表达（P＜0.05），同时显著诱导Cyp2d22蛋白的表达，但对Cyp3a11蛋白表达无明显作用，对Cyp2c37蛋白表达呈现显著抑制作用；中剂量（300 mg/（kg·d））能显著抑制Cyp3a11、Cyp2c37的mRNA和蛋白表达（P＜0.05），而对Cyp2d22的mRNA和蛋白表达无显著影响；高剂量（600 mg/（kg·d））对3 种酶的mRNA表达无显著影响，但对3 种酶的蛋白表达呈显著抑制作用（P＜0.05）。结果表明铁观音多酚提取物能够影响小鼠肝Cyp3a11、Cyp2d22和Cyp2c37的表达，揭示了铁观音多酚提取物与药物合用时产生的代谢性药物相互作用。

关键词: 铁观音茶, 多酚提取物, Cyp450, mRNA, 蛋白表达

Abstract: In this paper, the effect of polyphenols extracted from Tieguanyin tea on the mRNA and protein expression levels of cytochromes P450 3a11 (Cyp3a11), 2d22 (Cyp2d22) and 2c37 (Cyp2c37) in the liver of mice was explored by real-time reverse transcription polymerase chain reaction（real-time RT-PCR） and Western blot. The results showedthat the polyphenol extract at low dose (150 mg/(kg·d)) significantly induced the mRNA expression of Cyp3a11, Cyp2d22, and Cyp2c37 (P < 0.05) in comparison with the control group, while it significantly induced the protein expression of Cyp2d22 but had no significant effect on the protein expression of Cyp3a11 and exhibited a significant inhibitory effect on the protein expression of Cyp2c37. The polyphenols at middle dose (300 mg/(kg·d)) significantly inhibited the mRNA and protein expression of Cyp3a11 and Cyp2c37 (P < 0.05) but had no significant effect the mRNA and protein expression of Cyp2d22. The polyphenols at high dose (600 mg/(kg·d)), although having no significant effect on the mRNA expression of three enzymes, significantly inhibited their protein expression levels (P < 0.05). Therefore, Tieguanyin tea polyphenols can affect both the mRNA and protein expression levels of Cyp3a11, Cyp2d22 and Cyp2c37 in the liver of mice, suggesting that metabolism-based interactions can occur when they are used in combination with medicines.

Key words: Teiguanyin tea, polyphenol extracts, Cyp450, mRNA, protein expression

中图分类号:

O625.3

任红，刘冬，郑少杰，严弋敬，孙海燕，明建. 铁观音多酚提取物对小鼠肝Cyp450s的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201707032.

REN Hong, LIU Dong, ZHENG Shaojie, YAN Yijing, SUN Haiyan, MING Jian. Effect of Polyphenols Extracted from Tieguanyin Tea on the mRNA and Protein Expression Levels of Hepatic Cytochromes P450 (Cyp450s) in Mice[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201707032.

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铁观音多酚提取物对小鼠肝Cyp450s的影响

Effect of Polyphenols Extracted from Tieguanyin Tea on the mRNA and Protein Expression Levels of Hepatic Cytochromes P450 (Cyp450s) in Mice

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