食品科学 ›› 2018, Vol. 39 ›› Issue (8): 50-56.doi: 10.7506/spkx1002-6630-201808009

• 生物工程 • 上一篇    下一篇

响应面试验优化产琥珀酸放线杆菌GXAS137发酵粗甘油产丁二酸工艺

张红岩1,2,朱婧2,冯英2,3,李亿2,秦艳2,王青艳2,梁戈2,申乃坤2,4,*   

  1. (1.广西科学院生物研究所,广西?南宁 530007;2.广西科学院 非粮生物质酶解国家重点实验室,国家非粮生物质能源工程技术研究中心,广西?南宁 530007;3.沂水县科学技术局,山东?沂水 276400;4.广西民族大学海洋与生物技术学院,广西高校微生物与植物资源利用重点实验室,广西?南宁 530008)
  • 出版日期:2018-04-25 发布日期:2018-04-17
  • 基金资助:
    国家自然科学基金地区科学基金项目(31660022);广西自然科学基金项目(2016GXNSFBA380205); 南宁市科学研究与技术开发计划项目(20071004-1);广西民族大学相思湖青年学者创新团队项目(No.201706)

Optimization of Succinic Acid Production from Crude Glycerol by Actinobacillus succinogenes GXAS137 Using Response Surface Methodology

ZHANG Hongyan1,2, ZHU Jing2, FENG Ying2,3, LI Yi2, QIN Yan2, WANG Qingyan2, LIANG Ge2, SHEN Naikun2,4,*   

  1. (1. Biology Institute, Guangxi Academy of Sciences, Nanning 530007, China; 2. State Key Laboratory of Non-Food Biomass Energy and Enzyme Technology, National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China; 3. Yishui Science and Technology Bureau, Yishui 276400, China; 4. Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008, China)
  • Online:2018-04-25 Published:2018-04-17

摘要: 通过优化产琥珀酸放线杆菌GXAS137发酵粗甘油产丁二酸的培养基,提高丁二酸产量,降低生产成本。先通过单因素试验对粗甘油发酵产丁二酸的电子受体、初始粗甘油质量浓度及氮源进行了优化,再利用响应面试验确定重要参数的最佳水平。结果表明:二甲基亚砜(dimethyl sulfoxide,DMSO)为最适电子受体,玉米浆可替换酵母粉作为氮源,各因素的最佳条件为:初始粗甘油质量浓度55.43?g/L、DMSO质量浓度10.35?g/L、玉米浆质量浓度17.69?g/L。优化后丁二酸产量达到37.02?g/L,丁二酸得率为66.79%,生产强度为0.51?g/(L·h)。与初始条件下丁二酸产量(16.88?g/L)相比,优化后提高了1.19?倍。本研究为微生物发酵粗甘油原料生产丁二酸提供了理论支持。

关键词: 产琥珀酸放线杆菌GXAS137, 粗甘油, 丁二酸, 单因素试验, 响应面试验

Abstract: The optimization of medium components for improved succinic acid production by Actinobacillus succinogenes GXAS137 at reduced cost was performed using one-factor-at-a-time method and response surface methodology. The independent variables considered were electron acceptors, crude glycerol concentration and nitrogen sources. The results showed that dimethyl sulfoxide (DMSO) was the optimum electron acceptor and corn steep liquor (CSL) could be used as an alternative nitrogen source to replace yeast extract. The optimum culture medium that provided maximum succinic acid yield of 37.02 g/L, which was increased by 119% compared to that (16.88 g/L) before optimization, was composed of crude glycerol 55.43 g/L, DMSO 10.35 g/L and CSL 17.69 g/L. Under this optimized condition, the conversion rate of glycerol to succinic acid was 66.79% and succinic acid productivity was 0.51 g/(L·h). This study can provide a basis for the microbial production of succinic acid from crude glycerol.

Key words: Actinobacillus succinogenes GXAS137, crude glycerol, succinic acid, one-factor-at-a-time experiments, response surface methodology

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