食品科学 ›› 2011, Vol. 32 ›› Issue (16): 85-88.doi: 10.7506/spkx1002-6630-201116018

• 工艺技术 • 上一篇    下一篇

盐角草总黄酮提取工艺优化

赵梦丹1,2,王仁雷3,周 峰2,周泉澄2,周红霞2,华 春2,*,李建玲1   

  1. 1.南京师范大学生命科学学院 2.南京晓庄学院生物化工与环境工程学院 3.江苏教育学院生物系
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    江苏省高校自然科学基础研究面上项目(KJD180118);江苏省植物生理学精品课程建设项目(2010); 江苏省现代教育技术研究重点课题(2010)

Process Optimization for Extraction of Total Flavonoids from Salicornia europaea L.

ZHAO Meng-dan1,2,WANG Ren-lei3,ZHOU Feng2,ZHOU Quan-cheng2, ZHOU Hong-xia2,HUA Chun2,*,LI Jian-ling1   

  1. (1. College of Life Sciences, Nanjing Normal University, Nanjing 210046, China; 2. School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang College, Nanjing 211171, China; 3. Biology Department, Jiangsu Institute of Education, Nanjing 210013, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 采用分光光度法,以芦丁为标准品测定盐角草中的总黄酮含量。通过单因素试验分析乙醇体积分数、固液比、回流时间及回流温度4个主要因素对盐角草中总黄酮提取率的影响。在单因素试验的基础上通过正交设计法优化盐角草总黄酮提取工艺条件。结果表明:盐角草总黄酮的最佳提取工艺条件为乙醇体积分数80%、固液比1:50、回流温度70℃、提取时间4h。此条件下盐角草的总黄酮得率25.172mg/g。实验结果可能为今后盐角草资源的开发利用提供重要的理论依据。

关键词: 盐角草, 总黄酮, 索氏提取, 正交试验

Abstract: In this study, one-factor-at-a-time and orthogonal array design methods were used to optimize the conditions for hot reflux extraction of total flavonoids from the shoot of Salicornia europaea L. The determination of total flavonoids was spectrometrically carried out using rutin as the reference standard. The optimal conditions for extracting total flavonoids from the shoot of Salicornia europaea L. were 80% ethanol as the extraction solvent, solid/liquid ratio 1:50, extraction temperature 70 ℃, and extraction time 4 h, resulting in a total flavonoid yield of 25.172 mg/g.

Key words: Salicornia europaea L., total flavonoids, hot reflux extraction, orthogonal test

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