仿刺参肠道多糖的纯化及理化分析

doi:10.7506/spkx1002-6630-201117023

食品科学 ›› 2011, Vol. 32 ›› Issue (17): 118-122.doi: 10.7506/spkx1002-6630-201117023

仿刺参肠道多糖的纯化及理化分析

林威威1,2，张健2，王茂剑2,*，常耀光3，王共明1,2

1. 上海海洋大学食品学院 2. 山东省海洋水产研究所 3. 中国海洋大学食品科学与工程学院

发布日期:2011-08-30
基金资助:
国家海洋局海洋公益性行业科研专项(201105029)；　　　　　国家海洋局海洋经济规划科技推进平台与运作项目(国海科字[2007]219)

Purification and Physicochemical Analysis of Polysaccharides from Intestinal Tract of Apostichopus japonicus

LIN Wei-wei1,2， ZHANG Jian2， WANG Mao-jian2,*，CHANG Yao-guang3，WANG Gong-ming1,2

(1. College of Food Science & Technology, Shanghai Ocean University, Shanghai 200000, China； 2. Marine Fisheries Research Institute of Shandong Province, Yantai 264006, China； 3. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)

Published:2011-08-30

摘要/Abstract

摘要： 目的：对仿刺参肠道多糖进行纯化，并对纯化产物进行理化分析。方法：使用葡聚糖凝胶(G-100)层析柱分离出两种仿刺参肠道多糖，选取其中多糖A进行研究。使用葡聚糖凝胶(G-100)柱层析法和琼脂糖凝胶电泳法进行纯度检测，葡聚糖凝胶(G-200)柱层析测定分子质量，高效液相色谱法确定其单糖组分，并结合红外吸收光谱法与核磁共振对其结构进行初步检测。结果：实验所得纯化产物多糖A为均一组分，主要含有氨基糖醛酸、氨基半乳糖盐酸盐和岩藻糖3种单糖，并含有少量的氨基葡萄糖、半乳糖。其中岩藻糖有α和β两种构型，以α-岩藻糖硫酸酯残基、β-岩藻糖硫酸酯残基形式存在。分子质量约为18.8×104D。结论：该纯化产物多糖A为一种酸性多糖，推测其为硫酸软骨素类。

关键词: 肠道多糖, 纯化, 单糖分析, 结构鉴定, 海参, 仿刺参

Abstract: Objective: To purify and physicochemically analyze polysaccharides from the intestinal tract of Apostichopus japonicus. Methods: Two polysaccharides, named as polysaccharides A and B, respectively, were isolated and purified by using SephadexG-100 column from the intestinal tract of Apostichopus japonicus, and polysaccharide A was selected to study its physicochemical properties. Its purity was evaluated by SephadexG-100 column chromatography and agarose gel electrophoresis. The relative molecular mass was determined by Sephadex G-200 column chromatography. Monosaccharide components were analyzed by high performance liquid chromatography (HPLC) and their structures were identifiedby infrared spectroscopy and nuclear magnetic resonance. Results: The purified polysaccharide A was composed of GlcUA, GalN, Fuc, and a small amount of GlcN and Gal. Fucose had two configurations including the forms of α-fucose sulfate and β-fucose sulfate residues. This polysaccharide had a relative molecular mass of approximately 18.8 × 104 D. Conclusion: The purified polysaccharide A is an acidic polysaccharide, suggesting that it belongs to the chondroitin sulfate class.

Key words: intestinal tract polysaccharide, purification, component analysis, structure identification, Apostichopus japonicus

中图分类号:

TS254.1

林威威，张健，王茂剑，常耀光，王共明. 仿刺参肠道多糖的纯化及理化分析[J]. 食品科学, 2011, 32(17): 118-122.

LIN Wei-wei， ZHANG Jian， WANG Mao-jian，CHANG Yao-guang，WANG Gong-ming. Purification and Physicochemical Analysis of Polysaccharides from Intestinal Tract of Apostichopus japonicus[J]. FOOD SCIENCE, 2011, 32(17): 118-122.

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仿刺参肠道多糖的纯化及理化分析

Purification and Physicochemical Analysis of Polysaccharides from Intestinal Tract of Apostichopus japonicus

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