1 株水蜜桃采后病害拮抗细菌的鉴定及抑菌作用

doi:10.7506/spkx1002-6630-20171226-323

食品科学 ›› 2019, Vol. 40 ›› Issue (2): 102-109.doi: 10.7506/spkx1002-6630-20171226-323

1 株水蜜桃采后病害拮抗细菌的鉴定及抑菌作用

李培中1，许丽2，何惠霞3，尹京苑4，高海燕1,*

（1.上海大学生命科学学院，上海 200444；2.上海应用技术大学，上海 201418；3.上海佳迈农业技术服务有限公司，上海 201300；4.上海大学计算机工程与科学学院，上海 200444）

出版日期:2019-01-25 发布日期:2019-01-22
基金资助:
“十二五”国家科技支撑计划项目（2015BAD16B02）；国家自然科学基金青年科学基金项目（31401539）

Identification and Antifungal Activity of an Antagonistic Strain against Postharvest Disease in Honey Peach

LI Peizhong1, XU Li2, HE Huixia3, YIN Jingyuan4, GAO Haiyan1,*

(1. School of Life Sciences, Shanghai University, Shanghai 200444, China; 2. Shanghai Institute of Technology, Shanghai 201418, China; 3. Shanghai Jiamai Agricultural Technology Service Co. Ltd., Shanghai 201300, China; 4. School of Computer Engineering and Science, Shanghai University, Shanghai 200444, China)

Online:2019-01-25 Published:2019-01-22

摘要/Abstract

摘要： 为开发高效安全的生物保鲜剂，充分发掘和利用有益微生物资源，对1 株从豆腐乳中分离到的对水蜜桃采后病害具有较好防治效果的拮抗细菌CF-2，通过培养性状与形态特征、生理生化特征及16S rDNA同源序列比对分析进行鉴定，并测定其生长曲线及最适生长条件，同时采用平板对峙法及实体实验研究CF-2及其代谢产物对交链孢霉、桃褐腐菌、复端孢霉与丝核菌的抑制作用。结果表明：菌株CF-2经鉴定为枯草芽孢杆菌（Bacillus subtilis），培养24～48?h后生长最旺盛，最适生长pH值为7.0，最适生长温度为37?℃；CF-2对4?种病原真菌在体外均有抑制作用，平均抑制率为（38.70±9.22）%，其中对复端孢霉的抑制作用最大，抑制率为（52.30±3.67）%；其24?h发酵液及其无菌滤液与菌悬液对4?种病原菌亦有较强的抑制效果，其中无菌滤液对4?种病原真菌的平均抑制率为（68.76±5.77）%，对交链孢霉的抑制作用最大，抑制率为（77.41±1.91）%；经CF-2无菌滤液处理后，孢子萌发时间延长了62～64?h，12?d后孢子萌发率平均降低了87.93%；在实体实验中，25?℃室温下CF-2?24?h发酵液及无菌滤液与菌悬液可有效提升水蜜桃好果率，其中无菌滤液的效果最好，6?d时好果率提升了29%。以上结果表明，生防菌CF-2及其代谢产物对水蜜桃采后4?种病原菌有较强的抑制作用。

关键词: 拮抗细菌, 鉴定, 水蜜桃, 采后病害, 抑菌作用

Abstract: This study aimed to develop an effective and safe biological control agent and to sufficiently explore and make use of beneficial microorganisms. One bacterial strain named CF-2, which was separated from fermented bean curd and showed inhibitory effect on postharvest diseases of honey peaches, was identified based on morphological features, physiological and biochemical characteristics, and 16S rDNA sequence homology analysis. The growth curve of CF-2 and its optimal growth conditions were determined. Subsequently, the antimicrobial activity of CF-2 and its metabolites against four main postharvest fungal pathogens of honey peach (Alternaria alternata, Monilinia fructicola, Cephalothecium sp. and Rhizoctonia sp.) was assessed by plate confrontation method and in vivo experiments. The results showed that strain CF-2 was identified as Bacillus subtilis. At 24-48 h of culture, the growth rate of CF-2 reached a peak, and the optimal pH and temperature for its growth were determined as 7.0 and 37 ℃, respectively. CF-2 showed inhibitory effect on the four fungal pathogens in vitro with an average percentage inhibition of (38.70 ± 9.22)%, and it exhibited the strongest inhibitory effect on Cephalothecium sp. with a percentage inhibition of (52.30 ± 3.67)%. The 24 h fermented broth and its cell-free filtrate and cell suspension also had strong inhibitory effect on all four pathogenic fungi. The average percentage inhibition of the cell-free filtrate was (68.76 ± 5.77)% and it showed the strongest inhibitory effect on A. alternata with a percentage inhibition of (77.41 ± 1.91)%. Compared to the control group, treatment with the cell-free filtrate delayed spore germination of the fungal pathogens by 62-64 h and decreased spore?germination rate by 87.93% on average after 12 d. In in vivo experiments, the 24 h fermented broth of CF-2 and its cell-free filtrate and cell suspension all increased the percentage of marketable fruit compared to the control group, especially the cell-free filtrate, which showed the strongest effect, increasing the percentage of marketable fruit by 29% after 6 d. The above findings indicated that CF-2 and its metabolites had strong inhibitory effect on four main fungal pathogens in honey peach.

Key words: antagonistic bacteria, identification, honey peach, postharvest diseases, antifungal effect

中图分类号:

TS255.3

李培中，许丽，何惠霞，尹京苑，高海燕. 1 株水蜜桃采后病害拮抗细菌的鉴定及抑菌作用[J]. 食品科学, 2019, 40(2): 102-109.

LI Peizhong, XU Li, HE Huixia, YIN Jingyuan, GAO Haiyan. Identification and Antifungal Activity of an Antagonistic Strain against Postharvest Disease in Honey Peach[J]. FOOD SCIENCE, 2019, 40(2): 102-109.

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1 株水蜜桃采后病害拮抗细菌的鉴定及抑菌作用

Identification and Antifungal Activity of an Antagonistic Strain against Postharvest Disease in Honey Peach

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