食品科学 ›› 2020, Vol. 41 ›› Issue (16): 101-109.doi: 10.7506/spkx1002-6630-20190707-086

• 生物工程 • 上一篇    

风干肉中产脂肪酶瑞士乳杆菌TR13全基因组测序及序列分析

田建军,张开屏,赵艳红,李权威,马牧然,马俊杰,曹凯慧,靳烨   

  1. (1.内蒙古农业大学食品科学与工程学院,内蒙古 呼和浩特 010018;2.内蒙古商贸职业学院食品工程系,内蒙古 呼和浩特 010070)
  • 发布日期:2020-08-19
  • 基金资助:
    国家自然科学基金地区科学基金项目(31960514;31660439); “十三五”国家重点研发计划重点专项(2016YFE0106200);内蒙古自治区科技计划项目(2019GG239); 内蒙古自治区自然科学基金面上项目(2016MS0311;2017MS0363); 内蒙古自治区高等学校科学研究项目(NJZY17445)

Whole Genome Sequencing and Sequence Analysis of Lactobacillus helveticus TR13 from Air-dried Mutton

TIAN Jianjun, ZHANG Kaiping, ZHAO Yanhong, LI Quanwei, MA Muran, MA Junjie, CAO Kaihui, JIN Ye   

  1. (1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;2. Department of Food Engineering, Inner Mongolia Business and Trade Vocational College, Hohhot 010070, China)
  • Published:2020-08-19

摘要: 瑞士乳杆菌TR13是从自然风干羊肉中分离筛选到的1 株产脂肪酶活性高的菌株,为能够更好地研究该菌株在羊肉发酵过程中的代谢机理和功能,采用PacBio Illumina HiSeq测序平台对细菌TR13进行完成图测序分析。结果表明,瑞士乳杆菌TR13基因组为1 套环状无质粒分子,基因组序列总长度为2 172 224 bp,GC含量为36.82%。基因组中预测到2 536 个编码基因,编码基因总长度为1 883 532 bp,平均长度为799.46 bp,平均密度为1.08 个/kb,对应蛋白质平均序列长度为265 aa,占基因组百分比为86.71%。TR13菌株基因组中包含15 个rRNA操纵子和63 个tRNA。预测到可能的毒力基因75 个,耐药基因150 个。编码基因通过GO、COG和KEGG数据库的对比分析,完成了TR13菌株基因组基本功能注释和代谢通路基因信息注释,注释信息可为菌株应用研究提供一定理论依据。

关键词: 瑞士乳杆菌;全基因组测序;基因功能注释

Abstract: For a better understanding of its metabolic mechanism and function during mutton fermentation, the whole genome of Lactobacillus helveticus TR13, a strain with high lipase activity isolated from naturally air-dried mutton was sequenced using the PacBio Illumina HiSeq sequencing platform. The results showed that the genome of TR13 was a set of circular plasmid-free molecules, and the total length of the genome sequence was 2 172 224 bp with a GC content of 36.82%. It was predicted to contain 2 536 protein-coding genes with a total length of 1 883 532 bp, an average length of 799.46 bp, and an average density of 1.08 per kb, and the average sequence length of the coded proteins was 265 amino acids (aa), accounting for 86.71% of the genome. The genome of TR13 contained 15 rRNA operons and 63 tRNA, 75 virulence genes and 150 drug resistance genes being predicted from the genome. Moreover, functional annotation of the genome and metabolic pathway information annotation were accomplished by aligning the coding genes to the GO, COG and KEGG databases, which would provide a theoretical basis to explore the application of strain TR13.

Key words: Lactobacillus helveticus; whole genome sequencing; gene function annotation

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