食品科学 ›› 2020, Vol. 41 ›› Issue (20): 105-111.doi: 10.7506/spkx1002-6630-20190829-318

• 生物工程 • 上一篇    下一篇

盐渍辣椒细菌多样性分析

赵玲艳,黄嘉欣,杨剑,卿煜维,潘金微,周熠,邓放明   

  1. (1.湖南农业大学食品科学技术学院,湖南 长沙 410128;2.辣妹子食品股份有限公司,湖南 长沙 413100)
  • 出版日期:2020-10-25 发布日期:2020-10-23
  • 基金资助:
    国家特色蔬菜产业技术体系发酵加工岗位科学家项目(CARS-24-E-02);国家自然科学基金青年科学基金项目(31401675); 湖南省科技计划项目(2015NK3011);湖南农业大学“双一流”建设项目(SYL201802006); 湖南省企业科技创新创业团队支持计划项目(湘科人〔2018〕19号)

Analysis of Bacterial Diversity of Salted Peppers (Capsicum annuum)

ZHAO Lingyan, HUANG Jiaxin, YANG Jian, QING Yuwei, PAN Jinwei, ZHOU Yi, DENG Fangming   

  1. (1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Lameizi Food Co. Ltd., Changsha 413100, China)
  • Online:2020-10-25 Published:2020-10-23

摘要: 以盐渍线椒和盐渍米椒为研究对象,采用454焦磷酸测序技术,探究盐渍辣椒细菌的群落组成及相对丰度,为盐渍辣椒优势优良菌种的筛选及发酵辣椒菌种资源库的建立提供理论支持。结果表明,盐渍线椒已知分类的细菌可归属到8 个门、15 个纲、26 个目、31 个科、94 个属、101 个种。盐渍米椒已知分类的细菌可归属到6 个门、15 个纲、29 个目、44 个科、58 个属、42 个种。盐渍线椒主要的细菌门有蓝藻细菌(Cyanobacteria,77.01%)、变形菌门(Proteobacteria,14.78%)和厚壁菌门(Firmicutes,6.36%),盐渍米椒优势细菌门为Proteobacteria(51.5%)和Firmicutes(46.92%)。盐渍线椒和盐渍米椒相对丰度大于1%的细菌属共有19 个,盐渍线椒中优势菌属为乳球菌属(Lactococcus,24.51%)和根瘤菌属(Rhizobium,13.17%);盐渍米椒中优势菌属为盐厌氧菌属(Halanaerobium,38.99%)、海螺菌属(Marinospirillum,24.86%)、色盐杆菌属(Chromohalobacter,12.36%)和盐单胞菌属(Halomonas,9.67%)。

关键词: 盐渍线椒;盐渍米椒;454焦磷酸测序;细菌多样性

Abstract: The composition and relative abundance of the microbial community in salted peppers were explored by 454 pyrosequencing technology, aiming to provide a theoretical basis for the isolation and screening of superior strains from salted peppers for the production of pickled peppers. The results showed that the bacteria in salted Capsicium annuum L. var. dactylus M could be assigned to 101 species in 94 genera in 31 families in 26 orders in 15 classes in 8 phyla. The bacteria in salted C. annuum L. var. conoides Irish could be assigned to 42 species in 58 genera in 44 families in 29 orders in 15 classes in 6 phyla. The dominant phyla in salted C. annuum L. var. dactylus M were Cyanobacteria (77.01%), Proteobacteria (14.78%) and Firmicutes (6.36%). The dominant phyla in salted C. annuum L. var. conoides Irish were Proteobacteria (51.5%) and Firmicutes (46.92%). A total of 19 genera with relative abundance greater than 1% were found in both varieties. The dominant genera in salted C. annuum L. var. dactylus M were Lactococcus (24.51%) and Rhizobium (13.17%). The dominant genera in salted C. annuum L. var. conoides Irish were Halanaerobium (38.99%), Marinospirillum (24.86%), Halomonas (9.67%) and Chromohalobacter (12.36%).

Key words: salted Capsicium annuum L. var. dactylus M; salted Capsicium annuum L. var. conoides Irish; 454 pyrosequencing; bacterial diversity

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