泽泻不同部位药食功能成分分析比较

doi:10.7506/spkx1002-6630-20190919-250

食品科学 ›› 2020, Vol. 41 ›› Issue (20): 167-174.doi: 10.7506/spkx1002-6630-20190919-250

泽泻不同部位药食功能成分分析比较

蓝梦柳，杨利利，严桂杰，朱怀昌，李小艳，许文，吴水生

（1.福建中医药大学药学院，福建福州 350122；2.福建中医药大学生物医药研发中心，福建福州 350122）

出版日期:2020-10-25 发布日期:2020-10-23
基金资助:
国家自然科学基金面上项目（81773956；81872990）

Comparative Analysis of Functional Composition of Different Parts of Alisma orientale (Sam.) Juzep. for Medicinal and Food Purposes

LAN Mengliu, YANG Lili, YAN Guijie, ZHU Huaichang, LI Xiaoyan, XU Wen, WU Shuisheng

(1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; 2. Center of Biomedical Research & Development, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China)

Online:2020-10-25 Published:2020-10-23

摘要/Abstract

摘要： 目的：以泽泻不同部位（食用期泽泻花苔、泽泻叶、泽泻茎和药用泽泻块茎）作为研究对象，检测其药食功能成分包括总蛋白、总脂肪、氨基酸、总多糖、总黄酮、总多酚及5 种泽泻特有萜类成分（环氧泽泻烯、泽泻烯醇、泽泻醇B、23-乙酰泽泻醇B和11-去氧泽泻醇B）的含量并进行比较，为泽泻不同部位在功能保健及食品应用方面提供一定依据。方法：采用国家标准方法测定总氨基酸、总蛋白、总脂肪、总多酚的含量；采用硫酸-苯酚法和NaNO2-NaOH-Al(NO3)3紫外分光光度计法分别测定总多糖和总黄酮含量，采用超高效液相法测定环氧泽泻烯、泽泻烯醇、泽泻醇B、23-乙酰泽泻醇B和11-去氧泽泻醇B 5 个泽泻萜类成分的含量。结果：泽泻不同部位均含有总蛋白、总脂肪、氨基酸等所检测药食功能成分，其中泽泻花苔总蛋白、氨基酸、总脂肪和总多酚含量均高于其他部位（（328.0±19.1）、（219.7±10.5）、（48.0±11.5）mg/g和（17.63±0.60）mg/g），总多糖、总黄酮和活性萜类成分则在泽泻块茎中含量较高（（9.26±0.86）、（2.07±0.12）mg/g和（5.315±0.545）mg/g），泽泻茎、叶中各成分含量相对较低。结论：泽泻不同部位均含有药食功能成分，其中泽泻花苔富含各种营养成分，具有很大的开发价值。

关键词: 营养成分；活性成分；泽泻花苔；超高效液相色谱

Abstract: Objective: This study aimed to determine and compare the functional components (total protein, total fat, amino acids, total polysaccharides, total flavonoids and total phenolics as well as five unique anthraquinones, alismoxide, alismol, alisol B, alisol B 23-acetate and 11-deoxy-alisol B) for medicinal and food purposes of different parts (edible flower buds, leaves and stems; medicinal tubers) of Alisma orientale (Sam.) Juzep. in order to provide a basis for the application of these plant parts in health and functional foods. Methods: The contents of total amino acids, protein, fat and total polyphenols were determined by the methods specified in the national standards. The contents of total polysaccharides and total flavonoids were determined by the sulfuric acid-phenol method and the NaNO2-NaOH-Al(NO3)3 ultraviolet spectrophotometric method, respectively. Ultra-high performance liquid chromatography (UPLC) was applied to determine alismoxide, alismol, alisol B, alisol B 23-acetate and 11-deoxy-alisol B. Results: Each of the plant parts contained protein, fat, amino acids and other bioactive ingredients tested. The contents of total protein, amino acid, fat and polyphenol in flower buds were (328.0 ± 19.1), (219.7 ± 10.5), (48.0 ± 11.5), and (17.63 ± 0.60) mg/g) respectively, higher than those in the other parts. The contents of polysaccharides, flavonoids and bioactive terpenoids were higher in tubers, (9.26 ± 0.86), (2.07 ± 0.12), and (5.315 ± 0.545) mg/g), respectively. The content of each component in stems and leaves was relatively low. Conclusion: All tested parts of A.orientale contain bioactive ingredients for medicinal and food purposes. Its flower buds are rich in various nutrients and thus have great development potential.

Key words: nutrients; bioactive constituents; flower buds of Alisma orientale (Sam.) Juzep.; ultra-high performance liquid chromatography

中图分类号:

R917

蓝梦柳，杨利利，严桂杰，朱怀昌，李小艳，许文，吴水生. 泽泻不同部位药食功能成分分析比较[J]. 食品科学, 2020, 41(20): 167-174.

LAN Mengliu, YANG Lili, YAN Guijie, ZHU Huaichang, LI Xiaoyan, XU Wen, WU Shuisheng. Comparative Analysis of Functional Composition of Different Parts of Alisma orientale (Sam.) Juzep. for Medicinal and Food Purposes[J]. FOOD SCIENCE, 2020, 41(20): 167-174.

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泽泻不同部位药食功能成分分析比较

Comparative Analysis of Functional Composition of Different Parts of Alisma orientale (Sam.) Juzep. for Medicinal and Food Purposes

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