微波辅助萃取-超高效液相色谱-质谱法测定食品用纸容器中双酚A

doi:10.7506/spkx1002-6630-201416032

食品科学

微波辅助萃取-超高效液相色谱-质谱法测定食品用纸容器中双酚A

马晓斐1，宋炜2，梁天佐1，贾振国1，张岩1,*

1.河北省食品质量监督检验研究院，河北石家庄 050091；2.河北省食品药品检验院，河北石家庄 050011

出版日期:2014-08-25 发布日期:2014-08-25
通讯作者: 张岩
基金资助:
河北省质监局科技计划项目（2012ZD09）

Determination of Bisphenol A in Paper Food Containers by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry

MA Xiao-fei1, SONG Wei2, LIANG Tian-zuo1, JIA Zhen-guo1, ZHANG Yan1,*

1. Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050091, China;
2. Hebei Provincial Institute for Food and Drug Control, Shijiazhuang 050011, China

Online:2014-08-25 Published:2014-08-25
Contact: ZHANG Yan

摘要/Abstract

摘要：

建立食品用纸容器中双酚A的超高效液相色谱-质谱检测方法。采用微波辅助萃取仪对样品进行萃取，Sep-Pak C18固相萃取柱净化，甲醇-水（含有体积分数0.05%氨水）作为流动相，梯度洗脱，经Kinetex C18（2.1 mm×100 mm，2.6 μm）色谱柱分离，采用电喷雾电离，多反应监测模式下进行测定。结果表明：该方法在
1.0～100.0 μg/L质量浓度范围内线性良好，其相关系数（r²）为0.999 7。在5、10 μg/kg和50 μg/kg 3 个添加水平下，双酚A的平均回收率稳定在90.5%～98.6%之间，相对标准偏差均小于5.6%，方法检出限2.5 μg/kg。该方法操作简便，灵敏度高，适用于食品用纸容器中双酚A残留分析。

关键词: 超高效液相色谱-质谱, 双酚A, 微波辅助萃取, 食品用纸容器

Abstract:

A method for the determination of bisphenol A in paper food containers by ultra performance liquid
chromatography and tandem mass spectrometry (UPLC-MS-MS) after microwave-assisted extraction was developed.
Sample extracts were purification on a Sep-Pak C18 column. The target compound was separated on a Kinetex C18 (2.1 mm ×
100 mm, 2.6 μm) column using methanol-water (containing 0.05% ammonia water) as the mobile phase with gradient
elution, and detected by MS with electrospray ionization (ESI) under a multiple reaction-monitoring mode. In the range of
1.0 to 100.0 μg/L, the calibration curve developed showed good linearity with correlation coefficients (r²) of 0.999 7. The
recoveries for spiked samples were 90.5%–98.6% at three levels of 5, 10 and 50 μg/kg, and the relative standard deviations
were less than 5.6%. The limit of detection was 2.5 μg/kg. The method proved to be easy to use, timesaving, sensitive and
suitable for the determination of bisphenol A residues in food paper containers.

Key words: ultra performance liquid chromatography tandem mass spectrometry, bisphenol A, microwave-assisted extraction, paper food containers

中图分类号:

R917

马晓斐1，宋炜2，梁天佐1，贾振国1，张岩1,*. 微波辅助萃取-超高效液相色谱-质谱法测定食品用纸容器中双酚A[J]. 食品科学, doi: 10.7506/spkx1002-6630-201416032.

MA Xiao-fei1, SONG Wei2, LIANG Tian-zuo1, JIA Zhen-guo1, ZHANG Yan1,*. Determination of Bisphenol A in Paper Food Containers by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201416032.

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微波辅助萃取-超高效液相色谱-质谱法测定食品用纸容器中双酚A

Determination of Bisphenol A in Paper Food Containers by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry

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