食品科学 ›› 2020, Vol. 41 ›› Issue (10): 206-212.doi: 10.7506/spkx1002-6630-20190505-033

• 成分分析 • 上一篇    下一篇

超高效液相色谱-质谱联用技术解析黑果枸杞超临界CO2萃取物中黄酮类天然产物结构

束彤,丁丽娜,王茜,贾彩惠,邱亦亦,阮晖,,,   

  1. (1.青海省食品检验检测院,青海 西宁 810008;2.浙江大学生物系统工程与食品科学学院,浙江 杭州 310058;3.浙江省农产品加工技术研究重点实验室,浙江 杭州 310058;4.浙江大学宁波研究院,浙江 宁波 315100)
  • 出版日期:2020-05-25 发布日期:2020-05-15
  • 基金资助:
    浙江省科技计划项目(2017C26004);青海省科技计划项目(2017-ZJ-Y06);西宁市科技计划项目(2019-Y-09)

Structural Elucidation of Flavonoids in the Supercritical CO2 Extract of Black Goji Fruit by Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

SHU Tong, DING Lina, WANG Qian, JIA Caihui, QIU Yiyi, RUAN Hui,,,   

  1. (1. Qinghai Institute for Food Control, Xining 810008, China; 2. College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China; 3. Zhejiang Key Laboratory for Agro-food Processing, Hangzhou 310058, China; 4. Ningbo Research Institute, Zhejiang University, Ningbo 315100, China)
  • Online:2020-05-25 Published:2020-05-15

摘要: 以青海柴达木黑枸杞为研究材料,对其超临界CO2萃取物,采用超高效液相色谱-质谱联用技术和PeakView色谱工作站拟合进行分析,并通过二级质谱解析和紫外光谱及质谱数据比对,阐明其中的黄酮组分及其结构。共鉴定出黑果枸杞果的超临界CO2萃取物中含有13 种黄酮类化合物(7 种黄酮醇苷元及黄酮醇苷,3 种二氢黄酮醇苷元,2 种黄酮苷元和1 种二氢黄酮苷元),包括芦丁、槲皮素-3-β-葡萄糖醛酸苷、金丝桃苷即槲皮素-3-O-吡喃半乳糖苷、二氢槲皮素、二氢异鼠李素、二氢山柰酚、槲皮素、芹菜素、橙皮素、苜蓿素、山柰酚、异鼠李素和山柰素,其中橙皮素和苜蓿素在黑果枸杞成分研究中未被发现过。

关键词: 黑果枸杞, 黄酮, 超临界CO2萃取物, 超高效液相色谱-质谱联用

Abstract: The flavonoid components of the supercritical CO2 extract of black Goji berries from Qaidam, Qinghai were analyzed by ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) employing PeakView workstation, and their structures were determined by analysis of the tandem mass spectra, ultraviolet (UV) spectroscopic analysis and comparison of the mass spectral data with the reference standards. As a result, a total of 13 flavonoids were identified, including 7 flavonol aglycones and flavonol glycosides, 3 dihydroflavonol aglycones, 2 flavonoid aglycones and 1 dihydrogen, namely rutin, quercetin-3-β-glucuronide, hyperoside, quercetin-3-O-galactopyranoside, dihydroquercetin, dihydroiso rhamnosin, dihydrokaempferol, quercetin, apigenin, hesperetin, alizarin, kaempferol, isorhamnetin, and kaempferol. It is noteworthy that hesperetin and alizarin were found in black Goji for the first time.

Key words: black Goji, flavonoid, supercritical CO2 extract, ultra-high performance liquid chromatography coupled with tandem mass spectrometry

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