黑果枸杞多糖结构表征及对乙醇诱导胃黏膜上皮细胞损伤的影响

doi:10.7506/spkx1002-6630-20230619-146

食品科学 ›› 2024, Vol. 45 ›› Issue (9): 109-115.doi: 10.7506/spkx1002-6630-20230619-146

黑果枸杞多糖结构表征及对乙醇诱导胃黏膜上皮细胞损伤的影响

钱荣，安佳鑫，续晓琪，张涛，陈爱君，李莎，徐虹

（1.南京工业大学食品与轻工学院，江苏南京 211816；2.保龄宝生物科技有限公司，山东德州 251200；3.南京财经大学食品科学与工程学院，江苏省现代粮食流通与安全协同创新中心，江苏南京 210023）

出版日期:2024-05-15 发布日期:2024-05-01
基金资助:
国家自然科学基金青年科学基金项目（31801496）；国家自然科学基金面上项目（32372264）；江苏省自然科学基金项目（BK20210674）；江苏省高等学校基础科学（自然科学）项目（21KJB240003）；中国博士后基金面上项目（2021M690191）；山东省博士后创新项目（202102056）

Structural Characterization of a Water-Soluble Polysaccharide from Lycium ruthenicum Murr. Fruits and Its Protective Effect on Ethanol-Induced Damage in GES-1 Cells

QIAN Rong, AN Jiaxin, XU Xiaoqi, ZHANG Tao, CHEN Aijun, LI Sha, XU Hong

(1. College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; 2. Baolingbao Biology Co. Ltd., Dezhou 251200, China; 3. Modern Grain Circulation and Security Collaborative Innovation Center, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China)

Online:2024-05-15 Published:2024-05-01

摘要/Abstract

摘要： 采用热水浸提法提取黑果枸杞多糖（Lycium ruthenicum Murr. polysaccharide，LRMP），解析其单糖组成、糖苷键连接方式、链构象等结构参数，并评价其对乙醇诱导胃黏膜上皮细胞（gastric mucosal epithelial cells，GES-1）损伤的保护效果。结果表明，LRMP由阿拉伯糖、木糖、半乳糖、葡萄糖、岩藻糖和半乳糖醛酸组成，结构末端主要由阿拉伯糖、木糖、葡萄糖和半乳糖残基组成，在溶液中呈典型的无规卷曲构象，均方半径为（80.4±1.0）nm。LRMP对GES-1细胞无毒性作用，质量浓度600 μg/mL LRMP可以显著抑制乙醇对GES-1细胞造成的损伤，同时恢复细胞内超氧化物歧化酶的活性，降低GES-1细胞凋亡率。综上所述，LRMP作为功能性多糖在健康食品开发中具有潜力。

关键词: 黑果枸杞；多糖；结构；乙醇诱导损伤；胃黏膜上皮细胞

Abstract: Lycium ruthenicum Murr. polysaccharide (LRMP) was obtained by hot water extraction, its structural parameters such as monosaccharide composition, glycosidic linkages and chain conformation were analyzed, and its protective effect on ethanol-inducedinjuryin gastric mucosal epithelial cells (GES-1) was evaluated. The results showed that LRMP was composed of arabinose, xylose, galactose, glucose, fucose and galacturonic acid, and its terminus mainly consisted of arabinose, xylose, glucose and galactose residues. In aqueous solution, LRMP presented a typical random coil conformation with a mean square radius of (80.4 ± 1.0) nm. LRMP had no toxic effect on GES-1 cells, and 600 μg/mL LRMP could significantly ameliorate ethanol-induced damage, restore intracellular superoxide dismutase (SOD) activity, and reduce apoptosis rate in GES-1 cells. In conclusion, LRMP has potential application as a functional polysaccharide in the development of health foods.

Key words: Lycium ruthenicum Murr.; polysaccharide; structure; ethanol-induced damage; gastric mucosal epithelialcells

中图分类号:

TS204.1

钱荣，安佳鑫，续晓琪，张涛，陈爱君，李莎，徐虹. 黑果枸杞多糖结构表征及对乙醇诱导胃黏膜上皮细胞损伤的影响[J]. 食品科学, 2024, 45(9): 109-115.

QIAN Rong, AN Jiaxin, XU Xiaoqi, ZHANG Tao, CHEN Aijun, LI Sha, XU Hong. Structural Characterization of a Water-Soluble Polysaccharide from Lycium ruthenicum Murr. Fruits and Its Protective Effect on Ethanol-Induced Damage in GES-1 Cells[J]. FOOD SCIENCE, 2024, 45(9): 109-115.

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黑果枸杞多糖结构表征及对乙醇诱导胃黏膜上皮细胞损伤的影响

Structural Characterization of a Water-Soluble Polysaccharide from Lycium ruthenicum Murr. Fruits and Its Protective Effect on Ethanol-Induced Damage in GES-1 Cells

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