食品科学 ›› 2021, Vol. 42 ›› Issue (1): 172-179.doi: 10.7506/spkx1002-6630-20200119-246

• 营养卫生 • 上一篇    下一篇

姜黄素提高PC12细胞的抗氧化能力

肖宝平,陈露,曾珺,刘静雯,李健,李桂玲   

  1. (1.集美大学食品与生物工程学院,福建 厦门 361021;2.厦门市海洋功能食品重点实验室,福建 厦门 361021;3.福建省海洋功能食品工程技术研究中心,福建 厦门 361021)
  • 发布日期:2021-01-18
  • 基金资助:
    国家自然科学基金面上项目(31771972);福建省自然科学基金项目(2017J01447); 福建省自然科学基金青年创新项目(2019J05096);福建省省属高校科研专项基金资助项目(JK2017026)

Curcumin Enhances the Antioxidant Capacity of Pheochromocytoma (PC12) Cells

XIAO Baoping, CHEN Lu, ZENG Jun, LIU Jingwen, LI Jian, LI Guiling,   

  1. (1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; 2. Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China; 3. Engineering Technology Research Center for Marine Functional Food of Fujian Province, Xiamen 361021, China)
  • Published:2021-01-18

摘要: 目的:以低分化和高分化大鼠肾上腺嗜铬细胞瘤(pheochromocytoma,PC12)细胞为研究对象,探究姜黄素抗氧化能力及其可能的作用机理。方法:通过噻唑蓝法测定PC12细胞的相对增殖率,然后确定姜黄素的作用浓度及作用时间;采用2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐法及铁离子还原/抗氧化能力法测定细胞的总抗氧化能力(total antioxidant capacity,T-AOC);使用DCFH-DA荧光探针标记法测定活性氧(reactive oxygen species,ROS)水平;分别测定细胞超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力;采用实时荧光定量聚合酶链式反应法测定抗氧化酶基因的表达水平。结果:10 μmol/L姜黄素作用24 h后,低分化PC12细胞相对增殖率没有显著变化,但高分化细胞增殖受到显著抑制(P<0.05);经不同浓度姜黄素处理后,两种细胞的T-AOC均极显著提高(P<0.01),ROS水平极显著降低(P<0.01);进一步研究发现姜黄素对SOD、CAT和GSH-Px活力和基因表达均有一定的促进作用。结论:姜黄素可能通过提高SOD、CAT和GSH-Px的活力和其基因表达水平增强PC12细胞T-AOC、降低ROS水平,从而缓解氧化应激,维持细胞稳态。

关键词: 植物多酚;肾上腺嗜铬细胞瘤细胞;氧化应激

Abstract: Objective: Lowly and highly differentiated pheochromocytoma (PC12) cells were used to investigate the antioxidant capacity and underlying mechanism of curcumin. Methods: The relative proliferation rate of PC12 cells was measured by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, and the concentration and duration of curcumin treatment were determined. The effect of curcumin on the total antioxidant capacity (T-AOC) of PC12 cells was detected by 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) cation radical scavenging and ferric ion reducing antioxidant power (FRAP) methods. The level of reactive oxygen species (ROS) was measured by the dichloro-dihydro-fluorescein diacetate assay. The activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined using corresponding commercial kits, and the expression levels of antioxidant enzyme genes were detected by real-time fluorescent quantitative polymerase chain reaction. Results: Upon 10 μmol/L curcumin treatment for 24 h, the relative proliferation rate of lowly differentiated PC12 cells remained unchanged, while it was significantly inhibited in highly differentiated cells (P < 0.05). Treatment with curcumin at all concentrations tested significantly increased the T-AOC of both lowly and highly differentiated PC12 cells (P < 0.01), and reduced ROS level (P < 0.01). Furthermore, the activities and gene expression of SOD, CAT and GSH-Px were enhanced by curcumin. Conclusion: Curcumin can enhance the T-AOC, reduce ROS level, alleviate oxidative stress, and maintain cell homeostasis via increasing the activities and gene expression levels of SOD, CAT and GSH-Px.

Key words: plant polyphenols; pheochromocytoma cells; oxidative stress

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