苯乳酸和醋酸联用对大肠杆菌的抑菌机理

doi:10.7506/spkx1002-6630-20200128-285

食品科学 ›› 2021, Vol. 42 ›› Issue (3): 77-84.doi: 10.7506/spkx1002-6630-20200128-285

苯乳酸和醋酸联用对大肠杆菌的抑菌机理

宁亚维，付浴男，何建卓，侯琳琳，王志新，肖香，王世杰，贾英民

（1.河北科技大学食品与生物学院，河北石家庄 050018；2.北京工商大学食品与健康学院，北京 100048；3.江苏大学食品与生物工程学院，江苏镇江 212013）

发布日期:2021-02-25
基金资助:
河北省杰出青年科学基金项目（C2016208142）；中央引导地方科技发展专项（19942817G）

Antibacterial Mechanism of Phenyllactic Acid Combined with Acetic Acid on Escherichia coli

NING Yawei, FU Yunan, HE Jianzhuo, HOU Linlin, WANG Zhixin, XIAO Xiang, WANG Shijie, JIA Yingmin

(1. College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China; 2. School of Food and Health, Beijing Technology and Business University, Beijing 100048, China; 3. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)

Published:2021-02-25

摘要/Abstract

摘要： 以大肠杆菌为指示菌，研究了苯乳酸和醋酸的联合抑菌效应及作用机理。首先，联合抑菌指数法与时间-杀菌曲线对苯乳酸与醋酸的联合抑菌效应的评价结果显示，苯乳酸和醋酸对大肠杆菌具有协同抑菌效应。其次，Zeta电位分析表明苯乳酸和醋酸可以协同改变大肠杆菌细胞表面电荷分布。DiSC3(5)荧光探针标记荧光光谱数据显示苯乳酸和醋酸可以协同消散大肠杆菌细胞膜电势；荧光探针SYTO9/碘化丙啶（propidium iodide，PI）标记流式细胞术结合荧光显微镜结果显示，PI对1/4最小抑菌浓度（minimum inhibitory concentration，MIC）苯乳酸、1/2 MIC醋酸和1/4 MIC苯乳酸＋1/2 MIC醋酸联用组的沾染率分别为8.8%、1.6%、12.8%，表明苯乳酸与醋酸联用对细胞膜完整性具有协同破坏作用，但破坏程度较弱；扫描电子显微镜图表明苯乳酸和醋酸会导致大肠杆菌发生凹陷等形变。最后，荧光光谱法对基因组DNA的分析显示苯乳酸和醋酸可导致基因组DNA发生荧光猝灭现象，两者联用可增加猝灭程度，表明苯乳酸和醋酸对大肠杆菌基因组DNA具有协同破坏作用。综上所述，苯乳酸和醋酸两者可以通过协同改变细胞表面电荷分布，消散细胞膜电势，引起细胞发生形变，破坏基因组DNA从而发挥抑菌作用。

关键词: 苯乳酸；醋酸；抑菌；协同作用

Abstract: The synergistic antibacterial effect and underlying mechanism of phenyllactic acid (PLA) combined with acetic acid (ACE) were studied against Escherichia coli. Based on the fractional inhibitory concentration index and the time-killing curve, PLA and ACE exhibited a synergistic antibacterial effect. The zeta potential analysis suggested that PLA and ACE could change the distribution of bacterial surface charge. The fluorescence spectral data obtained using fluorophore DiSC3(5) demonstrated that they synergistically dissipated the membrane potential of E. coli on the results of flow cytometry and fluorescence microscopy using the?fluorophores SYTO9 and propidium iodide (PI) showed that PI staining rates of the bacterial cells treated with PLA at 1/4 minimum inhibitory concentration (MIC), ACE at 1/2 MIC, and their combination were 8.8%, 1.6%, and 12.8%, respectively, indicating that the PLA and ACE could synergistically but weakly damage the integrity of the cell membrane. Scanning electron microscopy observation demonstrated that PLA and ACE could result in sunken deformation of E. coli cells. Finally, fluorescence spectroscopic analysis indicated that PLA and ACE, especially their combination, could lead to fluorescence quenching of genomic DNA, suggesting that PLA and ACE could synergistically damage genomic DNA. Conclusively, PLA and ACE could exert synergistic antibacterial effect against E. coli by changing the distribution of surface charge on the cells, dissipating the membrane potential, deforming the cells, and disrupting genomic DNA.

Key words: phenyllactic acid; acetic acid; antibacterial; synergistic effects

中图分类号:

TS01.3

宁亚维，付浴男，何建卓，侯琳琳，王志新，肖香，王世杰，贾英民. 苯乳酸和醋酸联用对大肠杆菌的抑菌机理[J]. 食品科学, 2021, 42(3): 77-84.

NING Yawei, FU Yunan, HE Jianzhuo, HOU Linlin, WANG Zhixin, XIAO Xiang, WANG Shijie, JIA Yingmin. Antibacterial Mechanism of Phenyllactic Acid Combined with Acetic Acid on Escherichia coli[J]. FOOD SCIENCE, 2021, 42(3): 77-84.

苯乳酸和醋酸联用对大肠杆菌的抑菌机理

Antibacterial Mechanism of Phenyllactic Acid Combined with Acetic Acid on Escherichia coli

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