外源24-表油菜素内酯调控仔姜活性氧及酚类代谢减轻冷害

doi:10.7506/spkx1002-6630-20200223-256

食品科学 ›› 2021, Vol. 42 ›› Issue (3): 273-280.doi: 10.7506/spkx1002-6630-20200223-256

外源24-表油菜素内酯调控仔姜活性氧及酚类代谢减轻冷害

游玉明，汤洁，张美霞，王大平，陆红佳

（1.经济植物生物技术重庆市重点实验室，重庆 402160；2.重庆文理学院园林与生命科学学院，重庆 402160）

发布日期:2021-02-25
基金资助:
重庆市技术创新与应用发展项目（cstc2019jscx-msxmX0386）；重庆市教委科学技术研究项目（KJQN202001307）；国家级大学生创新创业训练计划项目（201910642005）

24-Epibrassinolide Alleviates Chilling Injury in Baby Ginger Rhizome by Regulating Active Oxygen and Phenolic Metabolism

YOU Yuming, TANG Jie, ZHANG Meixia, WANG Daping, LU Hongjia

(1. Chongqing Key Laboratory of Economic Plant Biotechnology, Chongqing 402160, China; 2. College of Landscape Architecture and Life Science, Chongqing University of Arts and Sciences, Chongqing 402160, China)

Published:2021-02-25

摘要/Abstract

摘要： 为探讨外源24-表油菜素内酯（24-epibrassionolide，EBR）处理对仔姜冷害的防控效果及可能机制，以‘竹根姜’为实验材料，采用外源EBR溶液浸泡30 min，晾干后置于4 ℃环境下贮藏25 d。定期分析冷害指数、硬度、相对电导率、H2O2含量、丙二醛含量、总酚含量、活性氧及酚类代谢相关酶活力等指标的变化。结果表明，10 μmol/L EBR处理能有效缓解仔姜的冷害症状，贮藏结束时，其冷害指数、ΔE分别为对照组的51.8%（P＜0.05）和48.1%（P＜0.01），硬度则比对照组高38.6%（P＜0.01）；相对电导率、丙二醛和H2O2含量分别比对照低24.6%、10.5%、24.8%（P＜0.05或P＜0.01），活性氧代谢相关的超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）和抗坏血酸过氧化物酶（ascorbate peroxidase，APX）活力分别比对照组高9.0%、35.7%和33.2%（P＜0.01），从而保护膜结构的完整性，减少活性氧的累积；同时，总酚含量及苯丙氨酸解氨酶（phenylalanine ammonia lyase，PAL）活力也分别比对照组高38.5%和24.8%（P＜0.01），而多酚氧化酶（polyphenol oxidase，PPO）活力则比对照组低17.3%（P＜0.01）。相关性分析显示，仔姜冷害指数与ΔE、相对电导率、丙二醛含量、SOD活力、PAL活力、PPO活力呈极显著正相关（P＜0.01），但与硬度、CAT活力及APX活力呈显著（P＜0.05）或极显著（P＜0.01）负相关。以上结果说明，外源EBR处理可通过调节仔姜活性氧及酚类代谢相关酶活性减轻氧化损伤，从而延缓其冷害的发生。

关键词: 仔姜；24-表油菜素内酯；冷害；活性氧；酚类物质

Abstract: In order to explore the effect of exogenous 24-epibrassinolide (EBR) treatment on preventing chilling injury (CI) and the possible underlying mechanism in baby ginger rhizome, ‘Zhugen’ ginger rhizomes were treated with EBR for 30 min, sun-dried and then stored at 4 ℃ for up to 25 days. Changes in CI index, firmness, relative electric conductivity, the contents of hydrogen peroxide (H2O2), malondialdehyde (MDA) and total phenolics, and enzyme activities associated with reactive oxygen species (ROS) and phenolic metabolism were analyzed regularly during the storage period. Results showed that 10 μmol/L EBR treatment distinctly alleviated CI symptoms during cold storage. At the end of storage, CI index and total color difference (ΔE) in the EBR-treated group were 51.8% (P < 0.05) and 48.1% (P < 0.01) as compared to the control group, respectively, and firmness was 38.6% higher than that in the control group (P < 0.01). Relative electric conductivity, MDA content and H2O2 content in EBR-treated group were decreased by 24.6%, 10.5% and 24.8%, respectively (P < 0.05 or 0.01), compared to the control group. The enzyme activities involved in ROS metabolism including superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were increased by 9.0%, 35.7% and 33.2% (P < 0.01), respectively, indicating that EBR can maintain cell membrane integrity and suppress the accumulation of ROS. Meanwhile, total phenolic content and phenylalanine ammonia lyase (PAL) activity were 38.5% and 24.8% higher in the EBR-treated group than in the control group, but polyphenol oxidase (PPO) activity was 17.3% lower than that in the control at the end of storage. Correlation analysis demonstrated that CI index was significantly positively correlated with ΔE, relative electric conductivity, MDA content, SOD, PAL and PPO activity (P < 0.01), but significantly negatively correlated with firmness, CAT and APX activity (P < 0.05 or 0.01). These results suggest that EBR could enhance chilling tolerance of baby ginger rhizome by regulating enzyme activities related to ROS and phenolic metabolism and ameliorating ROS-induced oxidative damage.

Key words: baby ginger rhizome; 24-epibrassionolide; chilling injury; reactive oxygen species; phenolics

中图分类号:

TS255.36

游玉明，汤洁，张美霞，王大平，陆红佳. 外源24-表油菜素内酯调控仔姜活性氧及酚类代谢减轻冷害[J]. 食品科学, 2021, 42(3): 273-280.

YOU Yuming, TANG Jie, ZHANG Meixia, WANG Daping, LU Hongjia. 24-Epibrassinolide Alleviates Chilling Injury in Baby Ginger Rhizome by Regulating Active Oxygen and Phenolic Metabolism[J]. FOOD SCIENCE, 2021, 42(3): 273-280.

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外源24-表油菜素内酯调控仔姜活性氧及酚类代谢减轻冷害

24-Epibrassinolide Alleviates Chilling Injury in Baby Ginger Rhizome by Regulating Active Oxygen and Phenolic Metabolism

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